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The Use of Giardia immunogenic Protein Fraction to Distinguish Assemblages in Humans and Animals

(2020) The Use of Giardia immunogenic Protein Fraction to Distinguish Assemblages in Humans and Animals. World's Veterinary Journal. pp. 421-428. ISSN 23224568 (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Buffalo calves have a high adverse effect on public health, specifically zoonotic Giardiasis in Egypt. The current study was carried out to investigate the use of enzyme-linked immunoelectrotransfer blot technique (EITB) as a preliminary step for the diagnosis of giardiasis before genotyping. For this purpose, fecal and blood samples were collected from diarrheic calves and workers in closed and non-closed beef buffalo farm. Zoonotic Giardia assemblage A was isolated from both diarrheic children and their close contact calves while the calves were infected by animal genotype assemblage E under low contact conditions. This was identified after the amplification and sequencing of a 292 bp fragment of 16S-rRNA ribosomal unit from 20 children and 28 calves Giardia isolates using nested PCR. Fractionation of different isolated Giardia assemblage and identification of specific fraction versus anti-Giardia-IgG antibodies in infected humans or animals using EITB revealed that α-1 giardin antigen with molecular weight ranged 29-34KDa. This specific immunogenic assemblage was fraction-related where it reacted specifically versus antibodies in sera of humans or animals infected by this assemblage and did not cross-react with giardin of other assemblage or with sera of non-infected cases. The results of the current study highlighted the use of EITB as a preliminary investigation before genotyping in the identification of zoonotic giardiasis.

Item Type: Article
Keywords: Buffalo calves, Genotype, Giardia, Human, Immunoblot, Nested PCR
Subjects: Q Science > Q Science (General)
S Agriculture > SF Animal culture
Divisions: World's Veterinary Journal (WVJ)
Page Range: pp. 421-428
Journal or Publication Title: World's Veterinary Journal
Journal Index: Scopus
Volume: 10
Number: 3
Publisher: Scienceline Publication, Ltd
Identification Number: https://doi.org/10.36380/scil.2020.wvj52
ISSN: 23224568 (ISSN)
Depositing User: Dr. Alireza Sadeghi
URI: http://eprints.science-line.com/id/eprint/508

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