(2020) The Use of Giardia immunogenic Protein Fraction to Distinguish Assemblages in Humans and Animals. World's Veterinary Journal. pp. 421-428. ISSN 23224568 (ISSN)
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WVJ 10(3) 421-428, Sep 25, 2020.pdf - Published Version Download (573kB) |
Abstract
Buffalo calves have a high adverse effect on public health, specifically zoonotic Giardiasis in Egypt. The current study was carried out to investigate the use of enzyme-linked immunoelectrotransfer blot technique (EITB) as a preliminary step for the diagnosis of giardiasis before genotyping. For this purpose, fecal and blood samples were collected from diarrheic calves and workers in closed and non-closed beef buffalo farm. Zoonotic Giardia assemblage A was isolated from both diarrheic children and their close contact calves while the calves were infected by animal genotype assemblage E under low contact conditions. This was identified after the amplification and sequencing of a 292 bp fragment of 16S-rRNA ribosomal unit from 20 children and 28 calves Giardia isolates using nested PCR. Fractionation of different isolated Giardia assemblage and identification of specific fraction versus anti-Giardia-IgG antibodies in infected humans or animals using EITB revealed that α-1 giardin antigen with molecular weight ranged 29-34KDa. This specific immunogenic assemblage was fraction-related where it reacted specifically versus antibodies in sera of humans or animals infected by this assemblage and did not cross-react with giardin of other assemblage or with sera of non-infected cases. The results of the current study highlighted the use of EITB as a preliminary investigation before genotyping in the identification of zoonotic giardiasis.
Item Type: | Article |
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Keywords: | Buffalo calves, Genotype, Giardia, Human, Immunoblot, Nested PCR |
Subjects: | Q Science > Q Science (General) S Agriculture > SF Animal culture |
Divisions: | World's Veterinary Journal (WVJ) |
Page Range: | pp. 421-428 |
Journal or Publication Title: | World's Veterinary Journal |
Journal Index: | Scopus |
Volume: | 10 |
Number: | 3 |
Publisher: | Scienceline Publication, Ltd |
Identification Number: | https://doi.org/10.36380/scil.2020.wvj52 |
ISSN: | 23224568 (ISSN) |
Depositing User: | Dr. Alireza Sadeghi |
URI: | http://eprints.science-line.com/id/eprint/508 |
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