Abstract

Brucellosis is one of the most important zoonotic diseases in the entire world. This disease results in serious economic loss and public health problems. The disease is caused by gram-negative bacteria of the genus Brucella. There is a need to perform control programs, such as conducting a vaccination program on livestock. One of the vaccine components that can be used is B. abortus lipopolysaccharide. The present study aimed to find out the effect of B. abortus lipopolysaccharide subunit vaccine in adjuvant Montanide ISA 70 against antibody titer and interferon-gamma (IFN- γ) level by administrating different doses and different post-vaccination sampling times. B. abortus lipopolysaccharide was used in the current study as an antigen and Montanide ISA 70 as an adjuvant. The samples were divided into three groups, each containing six sheep. In the control group (P0), the samples received no treatment. In the first treatment group (P1), the samples were subjected to the injection of B. abortus lipopolysaccharide subunit in Montanide ISA 70 adjuvant of 50 mg/ml. Regarding the second treatment group (P2), the samples had an injection of B. abortus lipopolysaccharide subunit vaccine in 100 mg/ml. The results showed that the administration of B. abortus lipopolysaccharide subunit vaccine in the Montanide ISA 70 adjuvant could influence the formation of antibodies and IFN-γ secretion on sheep. The administration of a dose of 100 μg/ml indicated a greater antibody titer, compared to the dose of 50 μg/ml. The administration of the vaccine at a dose of 50 μg/ml revealed a greater IFN-γ level value in comparison with the dose of 100 μg/ml. The result of the study on IFN-γ level indicated the control group had a greater IFN-γ level rather than the treatment group. In Conclusion, The administration of B. abortus lipopolysaccharide subunit vaccine in Montanide ISA 70 adjuvant could influence the formation of IFN-γ antibody and secretion on sheep.