(2020) Identifying the Virulent Factors of Clostridium perfringens Locally Isolated from Different Species. World's Veterinary Journal. pp. 617-624. ISSN 23224568 (ISSN)
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WVJ 10(4) 617-624, Dec 25, 2020.pdf - Published Version Download (455kB) |
Abstract
Clostridium perfringens incriminated in many diseases among different species of animals due to its ability to produce many virulence factors. In the current study, 135 intestinal samples were collected from different animal species of different localities in Egypt. Samples were subjected to isolation and identification (morphologically and biochemically) for obtaining Clostridium perfringens isolates (n=26, 19.25%). The PCR was carried out to elucidate the virulence factors. It was indicated that all the 26 Clostridium perfringens isolates had CPA gene and Clostridium perfringens enterotoxin (CPE gene), whereas 23% of isolates of chicken and cattle intestinal samples contained CPA, Net B, and CPE genes as virulence factors. Consequently, those isolates are highly recommended to be used in the preparation of enterotoxemia and necrotic enteritis vaccines as they are more virulent strains.
| Item Type: | Article |
|---|---|
| Keywords: | Clostridium perfringens, CPA gene, CPE gene, Net B gene |
| Subjects: | Q Science > Q Science (General) S Agriculture > SF Animal culture |
| Divisions: | World's Veterinary Journal (WVJ) |
| Page Range: | pp. 617-624 |
| Journal or Publication Title: | World's Veterinary Journal |
| Journal Index: | Scopus |
| Volume: | 10 |
| Number: | 4 |
| Publisher: | Scienceline Publication, Ltd |
| Identification Number: | https://doi.org/10.54203/scil.2020.wvj74 |
| ISSN: | 23224568 (ISSN) |
| Depositing User: | Dr. Alireza Sadeghi |
| URI: | http://eprints.science-line.com/id/eprint/415 |
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