(2019) Identification of locally isolated clostridium difficile from rabbits. World’s Veterinary Journal. pp. 1-7. ISSN 2322455X (ISSN)
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Abstract
Clostridium difficile is one of the most important pathogens causing diarrhea and enteritis in rabbits as it causes pseudomembranous colitis that leads to intestinal damage and deaths. In this study, screening of rabbit farms from different localities in Egypt had shown rabbits suffered from diarrhea and enteritis to detect Clostridium difficile by ELISA, it revealed that five out of 50 samples (10%) were positive for it. These samples were further identification by cultivation and culture characters, microscopical examination, agglutination test, pathogenicity test and Polymerase Chain Reaction (PCR) by using specific primers for toxins genes (tcdA and tcdB). The results showing that three out of five isolates were confirmed as Clostridium difficile and concluded that these isolates causing pseudomembranous enterocolitis in rabbits and this disease unable to be treated by antibiotics, so it used for preparation of vaccine against the disease in rabbits.
Item Type: | Article |
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Keywords: | Clostridiunm difficile, Rabbits, Enteritis |
Subjects: | Q Science > Q Science (General) S Agriculture > SF Animal culture |
Divisions: | World's Veterinary Journal (WVJ) |
Page Range: | pp. 1-7 |
Journal or Publication Title: | World’s Veterinary Journal |
Journal Index: | Scopus |
Volume: | 9 |
Number: | 1 |
Publisher: | Scienceline Publication, Ltd |
Identification Number: | https://doi.org/10.36380/SCIL.2019.WVJ1 |
ISSN: | 2322455X (ISSN) |
Depositing User: | Dr. Daryoush Babazadeh |
URI: | http://eprints.science-line.com/id/eprint/331 |
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