Abstract

This study was conducted to determine the effects of Bacillus subtilis DSM 32315 probiotic and antibiotic enramycin in broiler chickens with Clostridium perfringens induced-Necrotic enteritis on cecal microbial populations, functional diversity, nutrients transporters and cytokines mRNA expression. Day-old broilers (n= 360), Arbor Acre were randomly assigned to three dietary treatments such as control, basal diet fed-group only; antibiotic, basal diet plus enramycin 5 mg/kg; and probiotic group, basal diet plus Bacillus subtilis 2 x109 CFU/g. Antibiotic and probiotic fed groups was challenged with Clostridium perfringens at day1, and from day 14 to day 21. The results of present study showed that broiler chickens supplemented with antibiotic and probiotic significantly exhibited higher abundance of gut beneficial bacteria at the 21 and 35 days of age, while upregulated the expression of anti-inflammatory cytokine enterleukin-10 and secretory immunoglobulin-A. Expression of proinflammatory cytokines interleukin-6 tumor necrosis factor alpha, and interferon gamma were downregulated. Nutrient transporters of Peptide transporter-1, L amino transporter-2 and Cationic amino acid transporter-2 were upregulated in supplemented groups. More so, glucose transporter-2 Sodium glucose transporter-1, Solute carrier family 3, member 1, carbohydrates and vitamin metabolism cofactor enriched in probiotic fed-group, while control group exhibited up-regulation in interleukin-6, tumor necrosis factor alpha, and interferon gamma. Overall, supplementation of Bacillus subtilis DMS 32315 reduced the negative impact of necrotic enteritis in broiler chickens, and enhanced the gut-microbial community.