Scienceline Publication Repository

Scienceline Publication Repository

Scienceline Publication

Optimization of nutrient medium for Pediococcus acidilactici DS15 to produce GABA

(2019) Optimization of nutrient medium for Pediococcus acidilactici DS15 to produce GABA. Journal of World's Poultry Research. pp. 139-146. ISSN 2322455X (ISSN)

[img] Text
J World Poult Res 9(3) 139-146, 2019.pdf - Published Version

Download (504kB)

Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Nutrition is an essential factor for microorganisms to grow and survive. Carbon and nitrogen sources are used in producing primary and secondary metabolites. Gamma-Aminobutyric acid (GABA), a non-coded amino acid, is a secondary metabolite which acts as an inhibitory neurotransmitter of the central nervous system. Pediococcus acidilactici DS15 is a bacterium belonging to the order of Lactic Acid Bacteria. This study aimed to determine the effects of nutrients including glutamate, nitrogen and carbon sources on GABA production by Pediococcus acidilactici DS15. The tests were carried out using a range of 30 mM, 40 mM, 50 mM, 60 mM, and 70 mM glutamate as inducer and carbon sources in the form of peptone, yeast extract, skim milk, NH4NO3, KNO3, whey tofu and soy milk as nitrogen sources, and then glucose, lactose, maltosa, sucrose, palm, and cane sugar as carbon sources. The best sources of both will be tested with levels 10, 20, 30, 40, 50, 60, 70, 80, 90 and 100% for nitrogen and 1, 3, 5, 7, 9, 11, 13 and 15% for carbon. The results of this investigation revealed that the addition of 60 mM glutamate caused the higher amount of GABA production and the best source of nitrogen and carbon for Pediococcus acidilactici DS15 were 100% whey tofu and 15% palm sugar, respectively. Production rate of GABA by Pediococcus acidilactici DS15 could reach up to 311,485 mg / L.

Item Type: Article
Keywords: Carbon, GABA, Glutamate, Nitrogen, Pediococcus acidilactici DS15
Subjects: Q Science > Q Science (General)
S Agriculture > SF Animal culture
Divisions: Journal of World's Poultry Research (JWPR)
Page Range: pp. 139-146
Journal or Publication Title: Journal of World's Poultry Research
Journal Index: Scopus
Volume: 9
Number: 3
Publisher: Scienceline Publication, Ltd
Identification Number: https://doi.org/10.36380/jwpr.2019.17
ISSN: 2322455X (ISSN)
Depositing User: Dr. Daryoush Babazadeh
URI: http://eprints.science-line.com/id/eprint/114

Actions (login required)

View Item View Item