Abstract

The object of this study was to reduce the alginate content of Sargassum binderi by fermentation of Bacillus megaterium S245 in feed of laying hens. The experiment was arranged in a factorial with completely randomized design. The first factor was different inoculum dosage (1, 3, 5, 7, and 9%), while the second factor was different fermentation period (1, 3, 5, 7 and 9 days), and each treatment was replicated for five times. The measurements were total dry matter, organic matter, ash, alginate, and crude protein. The results showed there were no significant effect of inoculum dosage, and interaction between inoculum dosage and fermentation period on alginate, total dry matter, organic matter, ash and crude protein content of Sargassum binderi while fermentation period reduced the alginate and total dry matter content. Fermentation period also increased the crude protein content significantly. Besides that, fermentation period didn’t effect on organic matter and ash content significantly. The fermentation of Sargassum binderi with Bacillus megaterium S245 at inoculum dosage of 1% and fermentation period of nine days was the best combination for lowering alginate content in Sargassum binderi and this treatment had positive effect on nutrient content of Sargassum binderi.