Abstract

Bacterial infections, such as those caused by Pasteurella multocida serotype A, pose significant threats to poultry farming. The use of antibiotics to treat these infections can lead to antibiotic resistance. The present study aimed to identify Pasteurella multocida from 14 Hisex Brown layer chicken hen farms, with chikens aged 25–55 weeks, in West Java, Indonesia, and to evaluate their resistance to various antibiotics. Three samples from each farm were collected from dead chickens having symptoms of fowl cholera. Initially, the study involved isolating and identifying isolates from liver, heart, and lung organs via polymerase chain reaction. The colony was then tested for antibiotic resistance using the disk diffusion method. The results showed that 13 samples were Pasteurella multocida and nine were serotype A. The test results also indicated that all isolates were resistant to colistin (10 µg) and sensitive to tetracycline (30 µg), amoxicillin (25 µg), enrofloxacin (5 µg), sulfamethoxazole (25 µg), lincomycin (109 µg), and ciprofloxacin (5 µg). The study concluded that none of the Pasteurella multocida type A isolates were any longer sensitive to colistin, with some isolates still sensitive to tetracycline, amoxicillin, enrofloxacin, sulfamethoxazole, lincomycin, and ciprofloxacin, and two isolates showing multidrug resistance patterns. © The Author(s) 2024