Isolation and Molecular Characterization of Rabbit Haemorrhagic Disease Virus Strains Circulating in Rabbit Population Using Sequencing and Phylogenetic Analysis in upper Egypt

Abodalal, S. E. S. A. and Hafez, M. S. A. and Shosha, E. A. E. M. and Warda, F. F. and Hagag, N. M. (2021) Isolation and Molecular Characterization of Rabbit Haemorrhagic Disease Virus Strains Circulating in Rabbit Population Using Sequencing and Phylogenetic Analysis in upper Egypt. Journal of World's Poultry Research. pp. 302-311. ISSN 2322455X (ISSN)

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Abstract

Rabbit hemorrhagic disease (RHD) is a contagious viral disease that threatens rabbit farms locally and globally. The disease causative agent is the 63-Rabbit_Haemorrhagic_Disease_Virus_Strains_Circulating_in_Rabbit_Population_RHD virus (RHDV) of the family Caliciviridae. The present study aimed to identify and characterize RHDV strains currently circulating in Upper Egypt provinces. A total of 20 suspected RHDV samples were collected from non-vaccinated rabbit flocks from January to December 2019 in Upper Egypt governorates (New Valley and Assuit), Egypt. The RHDV was confirmed through the hemagglutination test (HA) and reverse transcription-polymerase chain reaction (RT-PCR). Further characterization of selected 4 isolates was performed by nucleotide sequencing of a partial VP60 gene. All of 11 RHDV RT-PCR-positive samples were positive for HA activity against human RBCs type "O". Based on the nucleotide sequencing, the selected 4 isolates were clustered as RHDV-1 variant strains (G3-G5). The nucleotide sequence identities of the 4 isolates were 94.2-100 %, compared to available RHDV strains from GenBank. In conclusion, the presence of RHDV-1 variant strains was detected and confirmed that threatens the rabbit’s populations in New Valley and Assuit governorates.

Item Type:	Article
Keywords:	Upper Egypt, Nucleotide sequencing, Rabbit hemorrhagic disease virus, Reverse transcription-polymerase chain reaction, VP60
Subjects:	Q Science > Q Science (General) S Agriculture > SF Animal culture
Divisions:	Journal of World's Poultry Research (JWPR)
Page Range:	pp. 302-311
Journal or Publication Title:	Journal of World's Poultry Research
Journal Index:	Scopus
Volume:	11
Number:	3
Publisher:	Scienceline Publication, Ltd
Identification Number:	https://doi.org/10.36380/jwpr.2021.37
ISSN:	2322455X (ISSN)
Depositing User:	Dr. Daryoush Babazadeh
URI:	https://eprints.science-line.com/id/eprint/89

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