The effect of Bacillus subtilis inoculum doses and fermentation time on enzyme activity of fermented palm kernel cake

Mirnawati, . and Ciptaan, G. and Ferawati, . (2019) The effect of Bacillus subtilis inoculum doses and fermentation time on enzyme activity of fermented palm kernel cake. Journal of World's Poultry Research. pp. 211-216. ISSN 2322455X (ISSN)

Text JWPR 9(4) 211-216, 2019 The Effect of Bacillus subtilis Inoculum Doses and Fermentation Time.pdf - Published Version Download (413kB)

Abstract

Palm kernel cake (PKC) was by-product of palm oil industry and it had potential to be one of the poultry ration ingredient However, its utilization for poultry was still limited because of the β-mannan in PKC. In order to increase PKC utilization in poultry ration, fermentation process was done to remodeled β mannan by using Bacillus subtilis. This research conducted a study on the effect of Bacillus subtilis inoculum dose and fermentation time to increase the enzyme activity of FPKC by using CRD with 3 × 3 factorial and 3 replications. Factor A was 3 doses of inoculum Bacillus subtilis: 3%, 5%, and 7%. Factor B was fermentation times which contained: (1) 2 days, (2) 4 days, and (3) 6 days. Parameters used were enzyme activity of mannanase, protease, and cellulase in FPKC. Significant interaction was seen between inoculum doses of Bacillus subtilis and fermentation time. There was also a significant interaction on each of the inoculums dose of Bacillus subtilis and fermentation time on all of the enzyme activity. This study concluded FPKC with Bacillus subtilis of 7% inoculums doses and 6 days fermentation time indicate the best result as seen from 24.27 U/ml of mannanase activity, 10.27 U/ml of protease activity, 17.13 U/ml of cellulose activity of fermented PKC.

Item Type:	Article
Keywords:	Bacillus subtilis, Enzyme activity, Fermentation time, Inoculum doses, Palm Kernel Cake
Subjects:	Q Science > Q Science (General) S Agriculture > SF Animal culture
Divisions:	Journal of World's Poultry Research (JWPR)
Page Range:	pp. 211-216
Journal or Publication Title:	Journal of World's Poultry Research
Journal Index:	Scopus
Volume:	9
Number:	4
Publisher:	Scienceline Publication, Ltd
Identification Number:	https://doi.org/10.36380/JWPR.2019.26
ISSN:	2322455X (ISSN)
Depositing User:	Dr. Daryoush Babazadeh
URI:	https://eprints.science-line.com/id/eprint/253

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