The Potential of Active Compounds in Gelinggang Leaf Extract as a Natural Antimicrobial for Laying Hens

Ariqoh, H and Retnani, Y and Hermana, W and Wijayanti, I and Firdaus, M and Taryati, . (2026) The Potential of Active Compounds in Gelinggang Leaf Extract as a Natural Antimicrobial for Laying Hens. Journal of World's Poultry Research. 68 - 75. ISSN 2322455X

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Abstract

Cassia alata L. (gelinggang) has been widely reported to contain bioactive secondary metabolites with antimicrobial properties. The advantages of utilizing Cassia alata L. encompass its antibacterial, antifungal, antioxidant, antiviral, antitumor, anti-inflammatory, antidiabetic, antihepatotoxic, and cytotoxic properties. This in vitro laboratory study aimed to evaluate the nutritional composition, phytochemical profile, and antibacterial activity of gelinggang leaf extract as a potential natural antimicrobial feed ingredient. Antibacterial activity was evaluated against Escherichia coli and Salmonella sp. using the agar well diffusion method. Proximate analysis revealed that Cassia alata leaf powder contained 20.35 crude protein, 10.05 crude fiber, 5.32 crude fat, 5.45 ash, and 51.44 nitrogen-free extract. Phytochemical screening confirmed the presence of alkaloids, flavonoids, saponins, tannins, phenolics, glycosides, triterpenoids, and steroids. The ethanol extract demonstrated moderate inhibitory antimicrobial activity against Escherichia coli and Salmonella sp. at concentrations ranging from 50 to 100; in contrast, lower concentrations exhibited weak activity inhibition. Cassia alata leaf extract demonstrated nutritional value with high protein content (20.35) and moderate in vitro antibacterial potential, indicating its promise as a natural antimicrobial candidate. © The Author(s) 2026. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/.

Item Type:	Article
Keywords:	Animal feed additive; Antibacterial activity; Bioactive compound; Cassia alata
Subjects:	S Agriculture > SF Animal culture
Divisions:	Journal of World's Poultry Research (JWPR)
Page Range:	68 - 75
Journal or Publication Title:	Journal of World's Poultry Research
Journal Index:	Scopus
Volume:	16
Number:	1
Publisher:	Scienceline Publication
Identification Number:	https://doi.org/10.36380/jwpr.2026.8
ISSN:	2322455X
Depositing User:	Dr. Daryoush Babazadeh
URI:	https://eprints.science-line.com/id/eprint/1771

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