Isolation and Identification of Antibiotic-Resistance Pathogenic Bacteria from Chicken Eggshells

Kartika, Aprilia Indra and Charanita, Febe Marchia and Fitria, Meutia Srikandi and Darmawati, Sri and Homsana, Anousin (2026) Isolation and Identification of Antibiotic-Resistance Pathogenic Bacteria from Chicken Eggshells. Journal of World's Poultry Research. 9 - 21. ISSN 2322455X

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Abstract

Chicken eggs represent a globally important source of animal protein for human consumption. Chicken eggs can serve as a source of bacterial contamination, including pathogenic bacteria resistant to antibiotics. Improper handling during purchase, storage, and cooking may allow pathogenic bacteria to infect consumers. The present study aimed to identify antibiotic-resistant pathogenic bacteria in chicken eggshells. This preliminary and exploratory study was carried out in several stages. These stages included collecting ten chicken eggs from five different stores in Semarang, Indonesia, performing bacterial culture, testing susceptibility to novobiocin and amoxicillin via the Kirby-Bauer method, performing Gram staining, and examining colony and cell morphology. The next stage included DNA extraction, 16S rRNA gene amplification, sequencing, and confirmation using morphological observations. The current results indicated that seven out of ten egg samples contained bacteria in their shells. Molecular identification revealed that bacterial isolates labeled A, B, C, D, E, F1, F2, G1, and G2, isolated from chicken egg, were closely related to Staphylococcus durrellii, Staphylococcus schleiferi, Streptococcus pyogenes, Streptococcus agalactiae, Staphylococcus aureus, Staphylococcus shinii, Staphylococcus saprophyticus, Staphylococcus cohnii, and Pseudomonas ficuserectae, respectively. Antibiotic resistance results demonstrated that samples A, B, C, F1, F2, and G2 were resistant to amoxicillin, while samples C and F2 were resistant to novobiocin. Chicken eggshells were found to include antibiotic-resistant pathogenic bacteria, mainly Staphylococcus and Streptococcus species, often resistant to amoxicillin. These current results indicated possible public health risks and highlight the importance of enhancing food safety measures. © The Author(s) 2026. Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.

Item Type:	Article
Keywords:	Antibiotic-resistant bacteria; Chicken eggshell; Contamination
Subjects:	S Agriculture > SF Animal culture
Divisions:	Journal of World's Poultry Research (JWPR)
Page Range:	9 - 21
Journal or Publication Title:	Journal of World's Poultry Research
Journal Index:	Scopus
Volume:	16
Number:	1
Publisher:	Scienceline Publication
Identification Number:	https://doi.org/10.36380/jwpr.2026.2
ISSN:	2322455X
Depositing User:	Dr. Daryoush Babazadeh
URI:	https://eprints.science-line.com/id/eprint/1764

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