Diversity Identification of Follicle Stimulating Hormone with PstI Restriction Enzyme in Female Mud Buffalo (Bubalus bubalis)

Afriani, Tinda and Khasrad, . and Jaswandi, . and Farhani, Faiza and Artha, Tiara Putri and Merdana, I Made (2026) Diversity Identification of Follicle Stimulating Hormone with PstI Restriction Enzyme in Female Mud Buffalo (Bubalus bubalis). World's Veterinary Journal. 278 - 286. ISSN 23224568

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Abstract

Low reproductive efficiency limits the productivity and milk yield in swamp buffaloes. Identifying follicle-stimulating hormone (FSH) is essential, as genetic variation in this gene can indicate reproductive potential. The present study aimed to characterize polymorphisms in the FSH gene, specifically exon 2, in female mud buffalo (Bubalus bubalis) using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) with the PstI restriction enzyme. Blood samples (n = 50) were collected through purposive sampling from healthy, mature female buffaloes (3-4 years of age, weighing 300-400 kilograms) across 50 farms in West Sumatra, Indonesia. Genomic DNA was extracted from the blood samples, and exon 2 of the FSH gene was amplified by PCR-RFLP, followed by PstI digestion to detect sequence variations. The PCR-RFLP analysis revealed two distinct genotypes, including a predominant homozygous genotype (+/+) in almost all buffaloes and a heterozygous genotype (+/-) observed in one sample. Additionally, one exhibited no visible DNA band. The allele frequencies for the allele with the PstI site (+) were 0.99, and the genotype distribution conformed to the Hardy-Weinberg equilibrium. The assessment of FSH gene diversity in Indonesian mud buffaloes confirmed that the exon 2 locus is polymorphic. These preliminary findings offered important insights for targeted breeding programs and promoting the conservation of local buffalo genetic resources. © The Author(s) 2026. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/.

Item Type:	Article
Keywords:	Follicle-stimulating hormone; Genotype; Mud buffalo; PstI enzyme
Subjects:	S Agriculture > SF Animal culture
Divisions:	World's Veterinary Journal (WVJ)
Page Range:	278 - 286
Journal or Publication Title:	World's Veterinary Journal
Journal Index:	Scopus
Volume:	16
Number:	1
Publisher:	Scienceline Publication
Identification Number:	https://doi.org/10.54203/scil.2026.wvj27
ISSN:	23224568
Depositing User:	Dr. Alireza Sadeghi
URI:	https://eprints.science-line.com/id/eprint/1697

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