Identification of Multidrug-Resistant Aeromonas salmonicida from Imported Seafood in Mosul City Shops via Microarray Technology

Albanna, Ayman and Al-Layla, Aseel Abdulmunem Hussein and AL-Saleem, Sahar Lukman (2026) Identification of Multidrug-Resistant Aeromonas salmonicida from Imported Seafood in Mosul City Shops via Microarray Technology. World's Veterinary Journal. 603 - 616. ISSN 23224568

Text WVJ16(2)603-616,2026.pdf - Published Version Download (1MB)

Abstract

The widespread emergence of multidrug-resistant (MDR) pathogens in imported seafood is an increasing public health concern. The present study aimed to detect Aeromonas salmonicida (A. salmonicida) in seafood samples and to assess the antibiotic resistance gene profiles of the isolated bacteria using conventional polymerase chain reaction (PCR) and DNA microarray analysis. Twenty samples consisting of shrimp, mussels, oysters, and salmon (five of each) were collected from shops in Mosul city, Iraq. The biochemical characteristics of the strains were determined using the VITEK 2 compact system, and their identities were confirmed by 16S rRNA gene sequencing, which showed �99 identity to database sequences. Nine samples tested positive for A. salmonicida, with a prevalence of 45 percentage, with shrimp and salmon each accounting for three isolates (60), mussels for two (40), and oysters for one (20). Microarray testing identified seven isolates as MDR, whereas two strains had no resistance genes and were antibiotic-sensitive. The current results revealed the absence of genes encoding extended-spectrum β-lactamases (ESBLs), including blaSHV, blaCTX-M, blaFOX, blaPER, blaVEB, and blaCMY, in all MDR strains. Tetracycline resistance was observed in 28.6 (2/7) of MDR isolates, with the tetA gene present, whereas the tetB gene was completely absent from all MDR isolates. Concerning sulfonamide resistance, all MDR isolates were found to carry sul1, whereas none carried sul2. The aphA and aadA1 genes were detected in all seven MDR strains, while the aac(6�)-Ib gene was found in only three of the seven MDR isolates (42.9). Additionally, each of these strains carried intI1 and qacE�1 genes. The widespread occurrence of MDR A. salmonicida carrying diverse resistance genes in imported seafood presented a significant public health risk. © The Author(s) 2026. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/.

Item Type:	Article
Keywords:	Aeromonas salmonicida; Microarray; Multidrug-resistant; Public health; Seafood
Subjects:	S Agriculture > SF Animal culture
Divisions:	World's Veterinary Journal (WVJ)
Page Range:	603 - 616
Journal or Publication Title:	World's Veterinary Journal
Journal Index:	Scopus
Volume:	16
Number:	2
Publisher:	Scienceline Publication
Identification Number:	https://doi.org/10.54203/scil.2026.wvj58
ISSN:	23224568
Depositing User:	Dr. Alireza Sadeghi
URI:	https://eprints.science-line.com/id/eprint/1681

Actions (login required)

View Item