Molecular Detection of Streptococcus equi subsp. Zooepidemicus and equi in Guinea Pig with Lymphadenitis by Targeting the comB and seeI Genes

Vargas, Diana Lizeth Arista and Solar, Jakson Jacob Socrates Chuquimia Del and Pablo, Jhorsan David Mauri and Enciso, Elthon Thomas Hinojosa and Quintana, Jorge Luis Maicelo and Lapa, Marco Rainer Lopez (2025) Molecular Detection of Streptococcus equi subsp. Zooepidemicus and equi in Guinea Pig with Lymphadenitis by Targeting the comB and seeI Genes. World's Veterinary Journal. 854 - 862. ISSN 2322-4568

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Abstract

Despite the economic and sanitary relevance of cervical lymphadenitis in guinea pigs, little molecular information is available regarding the role of Streptococcus (S..) equi subspecies in cervical lymphadenitis in Peru. The present study aimed to identify the subspecies S. equi subsp. zooepidemicus (SEZ) and S. equi subsp. equi (SEE) in guinea pigs (Cavia porcellus), with and without cervical lymphadenitis, from the Amazonas and Cajamarca regions of Peru, using PCR amplification of the sodA, comB, and seeI genes. A total of 50 guinea pigs, aged 6-8 months, were evaluated, 30 from Cajamarca (mean weight 1210.1 grams; 22 Peruvian breed, 1 Inti, and 7 Brown) and 20 from Amazonas (mean weight 950 grams; 10 Peruvian breed and 10 Inti), comprising 30 animals with cervical lymphadenitis and 20 clinically healthy animals. The sodA gene was first employed as a preliminary diagnostic marker, which confirmed the presence of S. equi in all animals presenting lymphadenitis. Subsequent amplification of the comB gene confirmed the exclusive presence of SEZ in all clinical cases, with no detection in the 20 clinically healthy guinea pigs analyzed in both study regions. In contrast, the seeI gene, specific to SEE, indicated no amplification in any of the samples, indicating the absence of SEE in the study population. The prevalence of SEZ (positive comB) among guinea pigs with cervical lymphadenitis was 100 in Amazonas (10/10; 95 CI: 69.2-100) and 100 in Cajamarca (20/20; 95 CI: 83.2-100), with no significant difference between regions. The present findings established SEZ as the primary etiological agent of cervical lymphadenitis in guinea pigs from both regions, with no molecular evidence of SEE. The present study underscored the utility of comB and seeI as specific and reliable molecular markers for differentiating SEZ and SEE. © The Author(s) 2025. Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article�s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article�s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/.

Item Type:	Article
Keywords:	comB gene; Guinea pig; seeI gene; Streptococcus equi subsp. equi; Streptococcus equi subsp. zooepidemicus
Subjects:	S Agriculture > SF Animal culture
Divisions:	World's Veterinary Journal (WVJ)
Page Range:	854 - 862
Journal or Publication Title:	World's Veterinary Journal
Journal Index:	Scopus
Volume:	15
Number:	4
Publisher:	Scienceline Publication
Identification Number:	https://doi.org/10.54203/scil.2025.wvj87
ISSN:	2322-4568
Depositing User:	Dr. Alireza Sadeghi
URI:	https://eprints.science-line.com/id/eprint/1676

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