Immunohistochemical distribution of HGF, IGF-I, FGF-2, and TGF-α in kidney and liver tissues of geese (Anser anser) during hatching and post-hatching periods

Ermutlu, Dilem Gülece and Aslan, �ahin (2025) Immunohistochemical distribution of HGF, IGF-I, FGF-2, and TGF-α in kidney and liver tissues of geese (Anser anser) during hatching and post-hatching periods. World's Veterinary Journal. 831 - 844. ISSN 2322-4568

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Abstract

Immunohistochemical studies during embryonic development of poultry are quite limited. The present study aimed to investigate the distribution of Hepatocyte growth factor (HGF), insulin-like growth factor-I (IGF-I), fibroblast growth factor-2 (FGF-2), and transforming growth factor-α (TGF-α) in the kidney and liver tissues of hatching and post-hatching goose via histologic and immunohistochemical methods. A total of 150 fertile goose eggs were used in the study. Throughout the incubation process (29 days), four eggs were used each day, starting from the sixth day, and the embryos were examined. The embryos were fixed in 10 formaldehyde solution. Geese at the 7, 15, 30, and 210 (adult) days after hatching were euthanized for a post-hatching evaluation. Routine histological procedures were performed on the kidney and liver tissues. For histological examinations were used Crossmonn�s triple staining, Periodic Acid-Schiff (PAS), and Haematoxylin and Eosin (H&E) staining. HGF, IGF-I, FGF-2, and TGF-α immunoreactivities were investigated in the histological slides. An examination of kidney tissue development indicated that metanephric blastema formation started on day 10. The mesonephros was replaced by the metanephros, the permanent kidney, starting on the day 25. An examination of liver tissue development revealed that sinusoids began to narrow, hepatic plaques formed, and endothelial and Kupffer cells became distinguished as early as day 8. Upon general evaluation, FGF-2 was found to be the growth factor with the most intense immunoreactivity in the liver and kidney, while IGF-I had the least immunoreactivity. There is a paucity of studies on growth factors during the embryonic period in poultry. In the present study, all four growth factors were immunohistochemically investigated in the kidney and liver during the hatching and post-hatching periods. Because poultry have a shorter embryonic period than mammals, they need to utilize growth factors effectively and at high levels. The present study demonstrated that HGF, IGF-I, FGF-2, and TGF-α growth factors were effective in kidney and liver development during the incubation period in geese. © The Author(s) 2025. Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article�s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article�s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/.

Item Type:	Article
Keywords:	Embryo; Goose; Growth factors; Kidney; Liver
Subjects:	S Agriculture > SF Animal culture
Divisions:	World's Veterinary Journal (WVJ)
Page Range:	831 - 844
Journal or Publication Title:	World's Veterinary Journal
Journal Index:	Scopus
Volume:	15
Number:	4
Publisher:	Scienceline Publication
Identification Number:	https://doi.org/10.54203/scil.2025.wvj85
ISSN:	2322-4568
Depositing User:	Dr. Alireza Sadeghi
URI:	https://eprints.science-line.com/id/eprint/1669

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