Quantitative Analysis of Small and Large Luteal Cells During Different Stages of Corpus Luteum Development in Holstein Cattle

Dung, Bui van and Thuy, Nguyen Thi Thanh and Anh, Nguyen Thi Ngoc and Nam, Nguyen Hoai (2025) Quantitative Analysis of Small and Large Luteal Cells During Different Stages of Corpus Luteum Development in Holstein Cattle. World's Veterinary Journal. 901 - 909. ISSN 2322-4568

Text WVJ15(4)901-909,2025.pdf - Published Version Download (1MB)

Abstract

The bovine corpus luteum (CL) undergoes dynamic structural and functional changes throughout its lifespan, driven by distinct populations of steroidogenic cells, including small luteal cells (SLCs) and large luteal cells (LLCs). Although these cell types have been morphologically characterized, quantitative evaluations of their numerical abundance and relative distribution across different developmental phases of the CL in cows remain limited. This study sought to quantify the populations of small and large luteal cells across the early, mid, and late stages of corpus luteum development in Holstein cows. Histomorphometric analysis of hematoxylin and eosin�stained ovarian sections was performed to provide insights into their temporal roles in luteal function. Ovarian samples were obtained from 30 Holstein cows (30 pairs), immediately after slaughter, and categorized into early, mid, or late luteal phases according to ovarian morphology and the appearance of corpora lutea. A total of 30 CL samples (10 per stage) were selected for evaluation. Each CL was fixed, paraffin-embedded, and sectioned longitudinally (5 μm thick) for histological examination. Small (<20 μm) and large (> 35 μm) luteal cells were quantified in standardized microscopic fields at � 40 magnification using ImageJ software. The results revealed that SLC counts were highest during the early luteal phase (72.53 ± 7.83) and significantly exceeded LLC counts (30.70 ± 3.78). In the mid-luteal phase, SLCs decreased (50.70 ± 2.82) while LLCs increased (44.30 ± 3.11), with no significant difference between them. During the late luteal phase, SLCs increased slightly (59.77 ± 3.84), whereas LLCs declined markedly (24.80 ± 2.52). Overall, SLCs counted were highest during the early luteal phase and declined toward the mid phase, while LLCs increased markedly at the mid luteal phase and decreased again at the late phase. © The Author(s) 2025. Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article�s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article�s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/.

Item Type:	Article
Keywords:	Corpus luteum; Histology; Large luteal cell; Small luteal cell
Subjects:	S Agriculture > SF Animal culture
Divisions:	World's Veterinary Journal (WVJ)
Page Range:	901 - 909
Journal or Publication Title:	World's Veterinary Journal
Journal Index:	Scopus
Volume:	15
Number:	4
Publisher:	Scienceline Publication
Identification Number:	https://doi.org/10.54203/scil.2025.wvj91
ISSN:	2322-4568
Depositing User:	Dr. Alireza Sadeghi
URI:	https://eprints.science-line.com/id/eprint/1666

Actions (login required)

View Item