Foot and Mouth Disease Virus Detection in Bali Cattle (Bos sondaicus) by RT-PCR in Lombok Island

Kholik, Kholik and Sukri, Akhmad and Wulan Ayu, Ieke and Vibrianita Nidom, Reviany and Indrasari, Setyarina (2025) Foot and Mouth Disease Virus Detection in Bali Cattle (Bos sondaicus) by RT-PCR in Lombok Island. World's Veterinary Journal. 1113 - 1119. ISSN 322-4568

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Abstract

Aphthovirus is responsible for foot-and-mouth disease (FMD) in cloven-hoofed animals, a highly infectious disease that has significant economic repercussions in various countries, including Indonesia. The present study aimed to use reverse transcription polymerase chain reaction (RT-PCR) to detect FMD in suspected Bali cattle in West Lombok, Indonesia. The current study was an observational, descriptive investigation conducted from July to August 2025, collecting 15 swab samples from male Bali cattle with an average weight of 210 kilograms and an average age of 2 years old. The samples were collected via purposive sampling from cattle demonstrating clinical signs of FMD, specifically from oral vesicular fluid. The samples were sourced from two smallholder farms in West Lombok; farm one, with 12 samples, and farm two, with three samples. The FMD was identified with a prevalence rate of 20PLACEHOLDER (3 out of 15), and a 328 bp DNA fragment was detected during gel electrophoresis. The current result indicated that the virus pathogen was detected in 20PLACEHOLDER of samples, and RT-PCR can be used as a high-sensitivity diagnostic method for this disease. © The Author(s) 2025. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article�s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article�s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/.

Item Type:	Article
Keywords:	Bali cattle; Foot-and-Mouth disease; PCR; Reverse transcription
Subjects:	S Agriculture > SF Animal culture
Divisions:	World's Veterinary Journal (WVJ)
Page Range:	1113 - 1119
Journal or Publication Title:	World's Veterinary Journal
Journal Index:	Scopus
Volume:	15
Number:	4
Publisher:	Scienceline Publication
Identification Number:	https://doi.org/10.54203/scil.2025.wvj114
ISSN:	322-4568
Depositing User:	Dr. Alireza Sadeghi
URI:	https://eprints.science-line.com/id/eprint/1654

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