EFFECTIVENESS OF Lactobacillus fermentum CMUL-54 AND Lactobacillus fermentum B978 AS PROBIOTIC CANDIDATES PRODUCING MANNANASE, CELLULASE AND PROTEASE ACTIVITIES FOR POULTRY

Iryos, Annisa Rahma and Mirnawati, Mirnawati and Harnentis, Harnentis and Srifani, Anifah and Yanti, Gusri (2025) EFFECTIVENESS OF Lactobacillus fermentum CMUL-54 AND Lactobacillus fermentum B978 AS PROBIOTIC CANDIDATES PRODUCING MANNANASE, CELLULASE AND PROTEASE ACTIVITIES FOR POULTRY. Online Journal of Animal and Feed Research. pp. 117-125. ISSN 2228-7701

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Abstract

The present research investigated the potential of Lactobacillus fermentum strains CMUL-54 and B978 as a probiotic candidates with mannanase, cellulase, and protease activities. The materials used in this research included L. fermentum CMUL-54, L. fermentum B978, MRS Broth containing oxgall, and various equipment and chemicals for analyzing probiotic candidates, mannanase, cellulase, and protease activities. This study utilized quantitative analysis conducted using a paired two-sample t-test with ten replications. The results revealed that L. fermentum CMUL-54 could be significantly (P<0.01) used as a probiotic candidate, showing resistance to temperatures of 42°C (9.9x109±0.71 CFU/ml), gastric pH (72.35±0.80), bile salt resistance (87.69±3.66), and hydrophobicity test to the intestine (92.40±0.30). Lactobacillus fermentum CMUL-54 also exhibited significant inhibitory zones against lactic acid bacteria (LAB) and pathogenic bacteria such as Escherichia coli (13.27±0.13mm), Salmonella enteritidis (13.91±0.13mm), Staphylococcus aureus (17.75±0.24mm), high activity mannanase (12.36±0.61U/ml), cellulase (12.42±0.24U/ml) and protease (11.30±0.08U/ml). It is concluded that L. fermentum CMUL-54 exhibited superior probiotic properties compared to L. fermentum B978, thus positioning it as a more promising candidate for improving broiler performance through enhanced digestion and overall health. © The Author(s) 2025

Item Type:	Article
Keywords:	Enzyme activity; L. fermentum B978; Lactobacillus fermentum CMUL-54; Probiotics
Subjects:	S Agriculture > SF Animal culture
Divisions:	Online Journal of Animal and Feed Research (OJAFR)
Page Range:	pp. 117-125
Journal or Publication Title:	Online Journal of Animal and Feed Research
Journal Index:	Scopus
Volume:	15
Number:	2
Publisher:	Scienceline Publication, Ltd
Identification Number:	https://doi.org/10.51227/ojafr.2025.14
ISSN:	2228-7701
Depositing User:	Dr. Saeid Chekani-Azar
URI:	https://eprints.science-line.com/id/eprint/1376

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