Abstract

Since the antibiotic growth promoter (AGP) banned, probiotics have become one of the increase relevant products to be developed in the poultry industry. Lactic acid bacteria (LAB) isolated from excreta have potential as probiotic. The aim of this study was to determine the potential probiotic properties of Lactobacillus and Lactococcus isolated from duck excreta. The LAB isolates were characterized by morphology, physiology, and biochemistry. Molecular identification by 16S rRNA analysis. LAB identification with API 50CHL and 16s rRNA showed Lactobacillus plantarum (L. plantarum) and Lactococcus lactis (Lc. lactis). The functional probiotic potential of the Lactobacillus species (L. plantarum BJ3) and Lactococcus species (Lc. lactis K5) was assessed by several parameters. The strains showed tolerance to variation of pH 2.0 to 5.0, and level bile salt 0.05 to 0.30%. The strain also showed the ability of intestinal adhesion and antibacterial activity against Escherichia coli. The strain was safe to use as a probiotic because it was negative for hemolytic activity and high sensitivity to antibiotics. A total of 100 Lohmann broilers strain) (7-day-old) were divided into 4 treatment groups; control (P0), L. plantarum BJ3 107 cfu/bird (P1), Lc. lactis K5 107 cfu/bird (P2), and L. plantarum BJ3 + Lc. lactis K5 107 cfu/bird (P3). Probiotic supplemented for chicken orally. Feed and water are provided ad libitum. Chickens are kept in a litter system. The result showed the probiotic supplementation, orally affected growth performance of broilers. Probiotic supplementation reduced feed intake (FI) during experimental period and the feed conversion ratio (FCR) were not affected. The P2 group (Lc. lactis K5 107 cfu/bird) showed the lowest FI. In conclusion, L. plantarum BJ3 and Lc. lactis K5 isolated from duck excreta can be considered as probiotics for chicken nutrition.