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Isolation of Newcastle Disease Virus from Wild Migratory Birds in Egypt

(2020) Isolation of Newcastle Disease Virus from Wild Migratory Birds in Egypt. Journal of World's Poultry Research. pp. 520-526. ISSN 2322455X (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Surveillance studies for Newcastle disease virus (NDV) are critical to monitor the potential spreading of these viruses among wild birds as well as domestic poultry. This study was conducted to determine the incidence of NDV in wild birds in Egypt in 2016. Out of 159 collected samples from eight different species of wild birds, six (3.77%) samples were positive for paramyxoviruses by semi-nested RT-PCR assay based on the RNA-dependent RNA polymerase gene. Of six positive samples, four NDVs were successfully isolated in 11-day-old specific-pathogen-free embryonated hens’ eggs. Partial sequences of the fusion gene of the four isolates were amplified using RT-PCR. Phylogenetic analysis of partial sequences of RNA-dependent RNA polymerase gene and fusion genes indicated that the detected NDV viruses in wild birds in Egypt are related to class I NDVs strains. Four Egyptian NDV isolates from wild birds exhibited sequence motif of 111GERQER↓LVG119 at the cleavage site as lentogenic virus in wild birds. Continuous active surveillance may help better monitoring of NDVs circulating in wild birds before newly emerging viruses in domestic poultry.

Item Type: Article
Keywords: Egypt, Fusion protein, Newcastle disease virus, Wild birds
Subjects: Q Science > Q Science (General)
S Agriculture > SF Animal culture
Divisions: Journal of World's Poultry Research (JWPR)
Page Range: pp. 520-526
Journal or Publication Title: Journal of World's Poultry Research
Journal Index: Scopus
Volume: 10
Number: 3
Publisher: Scienceline Publication, Ltd
Identification Number: https://doi.org/10.36380/jwpr.2020.60
ISSN: 2322455X (ISSN)
Depositing User: Dr. Daryoush Babazadeh
URI: http://eprints.science-line.com/id/eprint/211

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