%V 10 %D 2020 %N 2 %L eprints68 %I Scienceline Publication %T Prevalence of bovine gastro intestinal parasitic infection in and around kombolcha town %X A cross-sectional study was conducted in and around Kombolcha from October 2017 to April 2018 to determine the prevalence of gastro-intestinal helminthes parasites in cattle. A total of 384 randomly selected cattle were sampled and examined using standard coprological procedure. The overall prevalence was 39.8 of gastrointestinal (GI) helminthes and the prevalent helminthes eggs identified were 15.6 Paramphistomum species (spp), 10.4 strongly type eggs, 8.6 Fasciola spp., 3.1 Trichuris species and 2.1 Toxocaraspecies. This result indicated the highest prevalence of Paramphistomum spp. eggs than other helminthes egg and the lowest prevalence of Toxocara species egg. There was statistically significant difference among the age groups in paramphistomum and strongly infection (χ2=24.960, p≤0.001) and (χ2=17.047, p≤0.001) respectively. Higher prevalence rate was shown in 2-5 years age of cattle. Between body conditions there was also significant (p≤0.000 and p≤0.013) difference in paramphistomum and strongly and which was higher in moderate animals and lower in animals with good body condition. Sex had no significant effect on the prevalence of helminthes parasite, except for strongly type of egg. The present study revealed that there is high prevalence of GI helminthes infection in cattle in the study area. Therefore, strategic prevention should be advocated to prevent the problem in and around Kombolcha. © 2020. %R 10.36380/scil.2020.ojafr8 %K Cattle Gastrointestinal Helminthes parasites Kombolcha Prevalence %A A. Ayele %A M. Abay %A M. Birhan %A M. Yayeh %A M. Erara %A T. Gessese %A A. Mohammed %A G. Demoze %P 59-65 %J Online Journal of Animal and Feed Research %V 10 %D 2020 %N 3 %L eprints61 %I Scienceline Publication %T ISOLATION OF EXTRACELLULAR PHYTASE PRODUCING LACTIC ACID BACTERIA FROM THE GASTRO INTESTINAL TRACT OF POULTRY BIRDS %X Bacterial phytases and phytase-producing bacteria are of great industrial significance in the poultry industry and also in phosphorus pollution management. This study was designed to isolate and screen for phytase producing lactic acid bacteria from the duodenum, ileum and cecum of eight healthy cockerel samples. Standard microbiological procedures were followed to isolate phytase producing lactic acid bacteria using de Man Rogosa and Sharp (MRS) agar while extracellular phytase screening was done using phytase specific medium. The range of total microbial count obtain was highest at the cecum (2.85±0.11 to 4.34±0.12 log10 cfu/ml), lower at the duodenum (2.02±0.11 to 4.27±0.20 log10 cfu/ml) and lowest at the ileum (2.00±0.21 to 4.19±0.25 log10 cfu/ml). Nineteen bacterial isolates were identified as lactic acid bacteria on the basis of morphological, biochemical and physiological characterization and later identified as Lactobacillus species (78.94), Enterococcus species (15.78) and Lactococcus species (5.26). Thirteen out of the nineteen lactic acid bacteria showed phytase activity. Low phytase activity was observed in eight of the lactic acid bacteria isolates while five of the isolates produced significant extracellular phytase activity (>6mm). The most predominant Lactobacillus species were also found to be the most potent phytase producers. This can be exploited for industrial production of phytase in upgrading the nutritional status of feed and combating phosphorus pollution from poultry waste. © 2020, Online Journal of Animal and Feed Research. All rights reserved %R 10.36380/scil.2020.ojafr20 %K Gastrointestinal tract Lactic acid bacteria Phosphorus pollution Phytase Poultry industry %A A. A. Daodu %A G. D. Olumuyide %A L. Edemhanria %P 144-149 %J Online Journal of Animal and Feed Research