Scienceline Publication Repository: No conditions. Results ordered -Date Deposited. 2024-03-29T05:24:05ZEPrintshttp://eprints.science-line.com/images/Scienceline.pnghttp://eprints.science-line.com/2022-05-23T19:17:28Z2022-05-25T03:22:30Zhttp://eprints.science-line.com/id/eprint/367This item is in the repository with the URL: http://eprints.science-line.com/id/eprint/3672022-05-23T19:17:28ZAntibiotic profile of bacterial species isolated from broiler chickens with cellulitisHeaThe present study was carried out to isolate and identify the bacterial agents involved in field cases of avian cellulitis in broiler chickens and also to examine isolated bacteria for antibiotic susceptibility. The study was applied on 290 broiler chickens, aged 30-35 days, suffered from cellulitis (65 with head and 225 body lesions) to isolate bacterial agents. All obtained isolates were identified and tested for the pathogenicity based on Congo red assay. Disc diffusion test was used to study the sensitivity pattern of bacterial isolates with determination of multiple antibiotic resistance index. Results revealed that all head and 91.5% of body samples were positive on bacteriological examination. E. coli was the most prevalent isolate (45.2%), followed by staphylococci (33.2%), Clostridia (5.4%), streptococci (5.1%), Proteus mirabilis (4.4%), Enterobacter spp. (3.2%), Pseudomonas aeruginosa (2.2%), and Aeromonas spp. (1.2%). Congo red binding test was positive for P. aeruginosa (100%), Clostridia (72.7%), E. coli (65.8%), staphylococci (62.2%), Aeromonas spp. (60%), P. mirabilis (38.9%), Enterobacter spp. (38.5%) and streptococci (33.3%). Serological typing of E. coli identified nine O serotypes, with high predominance of O78 (19%). On antibiotic susceptibility profiling, E. coli isolates demonstrated 83.1-92.9% resistance to chloramphenicol, tetracycline, and enrofloxacin. Staphylococci isolates showed high resistance to ampicillin (97.0%) and clindamycin (82.9%). Clostridial and Aeromonas spp. isolates showed 100% resistant to tetracycline, enrofloxacin, and cefotaxime. Enterobacter spp. showed 100% resistance to chloramphenicol and cefotaxime. P. aeruginosa had 100% resistance to tetracycline and enrofloxacin. Also, streptococci isolates showed 100% resistance to erythromycin. Totally, 56.3% bacterial isolates were multidrug-resistant, 23.8% extensively drug-resistant and 1.5% pan drug-resistant. The present study concluded that E. coli is the most predominant pathogen involved in cellulitis, particularly O78 serotype. In addition, this study demonstrated high prevalence of multidrug-resistant bacteria among isolates, particularly against commonly used antibiotics. Therefore, it is recommended to use antibiotic sensitivity tests and accurate therapeutic doses to efficiently treat and control bacterial infections in poultry.M. M. Amerprofdramer@yahoo.comH. M. MekkyH. S. FedawyK. M. ElbayoumiD. M. Sedeek2022-05-23T19:17:21Z2022-05-25T03:22:38Zhttp://eprints.science-line.com/id/eprint/366This item is in the repository with the URL: http://eprints.science-line.com/id/eprint/3662022-05-23T19:17:21ZEffect of combined plant essential oils on dermanyssus gallinae: In vitro and in vivo studyThe present study was carried out to evaluate the effect of plant essential oils on Dermanyssus gallinae (D. gallinae). In vitro six groups of red mites, 20 mites in each group were exposed to direct spray of combined plant essential oils (Alisal) in rate of 0.25% on mites. activity and changes under stereomicroscope showed that sprayed mites completely stopped movements at both 1- and 2- h after treatment with completely stretched legs and white bead-like spots of oils accumulation on legs and bodies at 1- and 2- h. while, the non-treated mites were active with pale light brown colour. In vivo effect of Allisal to control red mite infestation in laying hens was investigated. In case of drinking water method mite count reduction rate on the bird was 60 %, 10%, and 0% as well as 0%, 0% and 10% in their traps at 4,7, and 12 days respectively, from the start of treatment in drinking water. While, in spray method mite reduction was 40%, 20%, and 10% on birds and 0%, 0% and 30% in the traps. On the other hand, water intake, feed intake, general health condition, skin health, and feather condition scores were improved at 4th day post treatment (DPT). Total lesion score at 12 DPT was improved. The present study concluded that in vitro combined plant essential oils have rapid and strong acaricide effect in contact sprays. In vivo, there was obvious improvement in groups treated with plant oils than non-treated group. Drinking water treated birds showed good results than spray treated group. Therefore, it is recommended to use combined plant essential oils in D. gallinae control strategies in poultry.A. M. M. Ameraziza.mahrous@gmail.comM. M. AmerH. M. MekkyH. S. Fedawy