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2024-03-29T12:37:09Z
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2022-10-16T11:13:27Z
2022-10-16T11:13:27Z
http://eprints.science-line.com/id/eprint/621
This item is in the repository with the URL: http://eprints.science-line.com/id/eprint/621
2022-10-16T11:13:27Z
QUALITY CHANGES IN SPERMATOZOA OF EXOTIC MUZAFFARNAGARI CROSS-BREED RAM SEMEN DURING THE STAGES OF FROZEN PRODUCTION
Good quality frozen semen is a prerequisite for successful artificial insemination. Changes in the
quality of sperm structure incur during semen processing of cryo-production. The current study, is, therefore,
designed to evaluate the Muzaffarnagari cross-breed ram sperm at the fresh diluted stage, pre-freeze, and
after freezing stages of frozen production. The semen collected was enlarged to include the A-part of 37°C
egg yolk media. After cooling to 5ºC for 2h, B-part of tris-citric acid egg yolk glycerol media added with Apart
and equilibrated at 5ºC for further 2h, transferred to 0.25 mL straws, placed in nitrogen vapor, frozen and
thawed and then analyzed. Sperm samples were assessed in fresh, pre-freezing, and post Thawing phases for
sperm motility, live sperm, ordinary sperm morphology, and intact sperm membrane. The mean percentage
of sperm motility at fresh diluted stage (81.67±0.93%) was decreased significantly (p<0.05) pre-freezing
stage (77.33±0.83%) because of refreshment and balance, and after freezing and thawing (44±2.45 percent)
decreased further (p<0.05). The Mean percentage of live sperm was higher (p<0.05) at fresh diluted stage
(91.27±0.57%) compared to pre-freeze (73.67±0.86%) and freezing stage (48.1±0.76%). The morphology
normal sperm reduced significantly (p<0.05) from fresh diluted stage (85.27±0.64%) to pre-freezing stage
(83.13±0.74). Their difference between the pre-freezing and the freezing stage is not significant (p>0.05)
(80.2±1.28 percent). The mean intact sperm membrane percentage decreased significantly (p<0.05) from
freshly diluted (82.4±0.77%) to pre-freezing (69.73±92%) and post-freezing (56.47±1.15%), respectively. The
percentage of intact acrosomes of spermatozoa also significantly (p<0.05) decreased from fresh stage
(95.8±0.39%) to pre-freeze (91.27±0.37%) and freezing stage (77.4±0.81%). In conclusion, during cryo-
freezing periods the sperm showed the highest motility, live and membrane damage.
M. Asaduzzaman
A. Saha
S. Akter
S. Biswas
M. G. S. Alam
F. Y. Bari
2022-01-16T23:36:23Z
2022-01-16T23:36:23Z
http://eprints.science-line.com/id/eprint/19
This item is in the repository with the URL: http://eprints.science-line.com/id/eprint/19
2022-01-16T23:36:23Z
COST COMPARISON OF ARTIFICIAL INSEMINATION AND NATURAL SERVICE IN SHEEP BREEDING SYSTEM
The cost information of animal breeding is a major concern in the farming system when making management and production system improvement decisions. Moreover, it is important to identify the most important cost positions and perspectives of the production process. This study is, therefore aimed to estimate, identify, and compare the cost contribution and breeding cost of artificial insemination (AI) vs. natural service in sheep production systems using frozen ram semen. Natural service by hand mating was performed to breed the ewes (n = 24) in estrus detected by a teaser ram maintained in a flock of 40 ewes. Artificial insemination was performed in synchronized ewes (n=10) after cervical ripening treatment using intramuscular injection of oxytocin. Breeding and performance costs were estimated by analyzing the cost associated factors. Per head insemination cost and cost per pregnancy in an artificial breeding program (2.80 and 5.59) were higher than natural breeding program (1.40 and 1.77) in sheep production. Ram depreciation cost, feed cost, and maintenance cost-shared the maximum cost in natural breeding, while the frozen semen cost, a special type of eccentric AI pipette cost, and hormone cost provoke the increased cost as the major constraints of the artificial breeding program. Minimizing the constraints and improving efforts in the conception rate of artificial insemination, farmers or producers will be benefited from natural breeding through rapid exploitation of the desired genetics cost-effectively. © 2021. All Rights Reserved.
M. Asaduzzaman
A. Saha
M. G. S. Alam
F. Y. Bari