TY  - JOUR
TI  - Interaction of Specific Monoclonal Antibodies with Leukocyte Antigens in Camels
SP  - 492
SN  - 2322-4568
EP  - 500
N1  - Department of Microbiology, College of Veterinary Medicine, King Faisal University, Al-Ahsa, Saudi Arabia; Department of Biological Sciences, College of Science, King Faisal University, Al Ahsa, 31982, Saudi Arabia
KW  - antigen CD206; CD3 antigen; CD5 antigen; CD8alpha antigen; CD9 antigen; leukocyte antigen; membrane antigen; monoclonal antibody; unclassified drug
KW  -  animal cell; antigen antibody reaction; antigen binding; antigen expression; antigen structure; Article; blood sampling; camel; cell subpopulation; controlled study; cross reaction; flow cytometry; granulocyte; human; human cell; immune system; immunocompetent cell; immunofluorescence; male; monocyte; mouse; nonhuman; staining
AV  - public
A1  - Alalai, M.A.
A1  - Alkuwayti, M.A.
A1  - Alrabiah, N.A.
A1  - Hussen, J.
IS  - 4
PB  - SCIENCELINE
JF  - World's Veterinary Journal
VL  - 13
Y1  - 2023/12/25/
ID  - eprints956
N2  - The dromedary camel as a livestock species significantly impacts the economy of arid and semi-arid regions worldwide. The identification of cross-reactive antibodies against pivotal immune cell markers acts as a valuable method to investigate the immune system of camels. The aim of the present study was to identify new monoclonal antibodies that react with camel leukocyte subsets using flow cytometry and multicolor immunofluorescence. The expression patterns of the tested antibodies indicated cross-reactivity of the anti-bovine CD9 monoclonal antibody clones LT86A and Hl9a with different binding potential. Although all leukocyte subpopulations stained positively with the CD9 antibodies, monocytes showed the highest CD9 abundance, compared to lymphocytes and granulocytes. No cross-reactivity was identified for the tested monoclonal antibodies against equine CD8a (clone: ETC142BA1), mouse CD3 (clone: CD3-12), human CD3 (clone: T3/2/16A9), human CD206 (clone: MMR), and bovine granulocytes (clone: CH138A). The present study revealed that only camel monocytes showed positive staining with the anti-ovine CD5 mAb (clone ST1), which is in contrast to the human and murine systems. The present findings indicated low homogeneity between camels and other species in the antigenic structure of leukocyte antigens, highlighting the need to develop camel-specific mAbs against the main immune cell markers. © The Author(s) 2023. All Rights Reserved.
UR  - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85184745190&doi=10.54203%2fSCIL.2023.WVJ52&partnerID=40&md5=3287c10fdb58c2027d6e95106b7f7ecb
ER  -