<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Carrageenan-Induced Acute Inflammation on Back-Skin of Mice: Histopathological Features, Number of Inflammatory Cells, and Expression of COX-2, COX-1, and IL-6"^^ . "Carrageenan is a sulfated polysaccharide obtained from red seaweed (Rhodophyceae) and can trigger inflammatory activation in both humans and laboratory animals. This study aimed to investigate the expression of cyclooxygenase-2 (COX-2), cyclooxygenase-1 (COX-1), and interleukin-6 (IL-6) and the number of inflammatory cells (neutrophil) involved in a carrageenan-induced acute inflammatory model in the back skin of mice. Paraffin blocks from the back skin of female Swiss mice aged 8 weeks were used in this study. The back-skins of 4 groups of 5 mice in each group were subcutaneously injected with 1, 2, and 4 carrageenan powder in 0.9 buffer saline and 0.9 buffer saline as control. Skin samples on paraffin blocks were taken 6 hours after carrageenan injection. Furthermore, paraffin blocks were stained with hematoxylin-eosin (HE) to count the number of inflammatory cells. Immunohistochemistry staining using anti-COX-2, COX-1, and IL-6 antibodies was performed to determine the role of inflammatory mediators. The results showed that the number of inflammatory cells (neutrophils) increased significantly following an increase in carrageenan concentrations. The COX-2, COX-1, and IL-6 expressed by inflammatory cells increased significantly at carrageenan concentrations of 1 to 4. Histopathological features supported the results obtained from the calculation of the number of inflammatory cells and the expression of COX-2, COX-1, and IL-6. The inflammatory markers consisting of COX-2, COX-1, and IL-6 were expressed on the back skin of mice at 6 hours post-injection with 1 to 4 carrageenan. It can be concluded that carrageenan can be used for an acute inflammatory model of the back skin of a mouse. This inflammation model is intended to facilitate the evaluation or measurement of therapeutic and inflammatory responses when test substances are administered topically or transdermal. © The Author(s) 2023. All Rights Reserved."^^ . "2023-12-25" . . "13" . "4" . . "SCIENCELINE"^^ . . . "World's Veterinary Journal"^^ . . . "23224568" . . . . . . . . . . . . . . . . "A.D."^^ . "Paryuni"^^ . "A.D. Paryuni"^^ . . "S."^^ . "Widyarini"^^ . "S. Widyarini"^^ . . "A.M."^^ . "Akrom"^^ . "A.M. Akrom"^^ . . "S"^^ . "Sugiyono"^^ . "S Sugiyono"^^ . . . . . . "Carrageenan-Induced Acute Inflammation on Back-Skin of Mice: Histopathological Features, Number of Inflammatory Cells, and Expression of COX-2, COX-1, and IL-6 (Text)"^^ . . . . . "WVJ13(4),520-530,December25,2023.pdf"^^ . . "HTML Summary of #954 \n\n\n \n Carrageenan-Induced Acute Inflammation on Back-Skin of Mice: Histopathological Features, Number of Inflammatory Cells, and Expression of COX-2, COX-1, and IL-6\n \n\n" . "text/html" . . . "SF Animal culture"@en . .