eprintid: 950
rev_number: 7
eprint_status: archive
userid: 5
dir: disk0/00/00/09/50
datestamp: 2024-04-29 14:49:25
lastmod: 2024-04-29 14:49:25
status_changed: 2024-04-29 14:49:25
type: article
metadata_visibility: show
creators_name: Prakoso, Y.A.
creators_name: Sigit, M.
creators_name: Aliviameita, A.
creators_id: yos.vet.docter@gmail.com
creators_orcid: 0000-0003-4908-6661
creators_orcid: 0000-0003-0685-7593
creators_orcid: 0000-0003-3027-892X
title: Standardization of the Simple Methodology for Experimentally Induced Ischemic Stroke in Rat Models
ispublished: pub
subjects: SF
divisions: j13
full_text_status: public
keywords: Common carotid artery; Ischemic stroke; Ligation; Rat model; Standardization
note: Department of Pharmacology, Faculty of Veterinary Medicine, University of Wijaya Kusuma Surabaya, Surabaya, Indonesia; Department of Reproduction, Faculty of Veterinary Medicine, University of Wijaya Kusuma Surabaya, Surabaya, Indonesia; Laboratory of Clinical Pathology, Faculty of Health Sciences, University of Muhammadiyah Sidoarjo, Sidoarjo, Indonesia
abstract: Stroke is a globally significant and devastating disease that requires prompt treatment. Animal models are commonly used to investigate stroke therapy, often through experimentally induced ischemic stroke (EIIS). However, challenges arise in implementing EIIS in animal models. The current study aimed to present a simple EIIS methodology for animal models. A total of 60 male Sprague-Dawley rats were randomly divided into five groups, namely Group 1 (sham-operated), Groups 2 to 5 (EIIS groups) with different duration of common carotid artery (CCA) ligation, including 1, 2, 4, and 8 hours, respectively. The ligation was performed on the CCA and its branches. Before the experiment, the rats were anesthetized, and the incision area was shaved and disinfected. The sagittal ventral midline was incised, with neck muscles retracted to expose the right CCA. The occlusion was performed on three sides of a carotid artery (common, external, and internal) using a simple interrupted suture. The occlusion of blood flow using ligation was performed at different times depending on the groups. After that, the CCA ligations were re-perfused by cutting the suture knot. The brain and blood were collected on days 1 and 7 after reperfusion. The results indicated that 4 and 8 hours of CCA ligation significantly impacted the general condition and neuro-deficit score. Moreover, 4 and 8 hours of CCA ligation could induce ischemic stroke by its capacity to cause infarction within the brain parenchyma and increase the platelet-to-white blood cell ratio, C-reactive protein, and De Ritis ratio. In contrast, 1 and 2 hours of CCA ligation did not significantly affect the observed parameters. It can be concluded that the EIIS using 4 and 8 hours of CCA ligation can be applied to induce ischemic stroke in rat models with consistent impacts on general conditions, neuro-deficit, hematology, and serology. © The Author(s) 2023. All Rights Reserved.
date: 2023-12-25
publication: World's Veterinary Journal
volume: 13
number: 4
publisher: SCIENCELINE
pagerange: 510-519
id_number: 10.54203/SCIL.2023.WVJ54
refereed: TRUE
issn: 2322-4568
official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85184764894&doi=10.54203%2fSCIL.2023.WVJ54&partnerID=40&md5=076ae1c9687ed0155c7dcb07b9f20b5a
j_index: scopus
citation:    (2023)     Standardization of the Simple Methodology for Experimentally Induced Ischemic Stroke in Rat Models.   World's Veterinary Journal.  pp. 510-519.  ISSN 2322-4568   
document_url: http://eprints.science-line.com/id/eprint/950/1/WVJ13%284%29%2C510-519%2CDecember%2025%2C2023.pdf