TY  - JOUR
UR  - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85119191486&doi=10.36380%2fjwpr.2021.37&partnerID=40&md5=71198154c474d5434f55c19f88691ab4
SP  - 302
Y1  - 2021/09/25/
AV  - public
A1  - Abodalal, S. E. S. A.
A1  - Hafez, M. S. A.
A1  - Shosha, E. A. E. M.
A1  - Warda, F. F.
A1  - Hagag, N. M.
KW  - Upper Egypt
KW  -  Nucleotide sequencing
KW  -  Rabbit hemorrhagic disease virus
KW  -  Reverse transcription-polymerase chain reaction
KW  -  VP60
EP  - 311
VL  - 11
SN  - 2322455X (ISSN)
TI  - Isolation and Molecular Characterization of Rabbit Haemorrhagic Disease Virus Strains Circulating in Rabbit Population Using Sequencing and Phylogenetic Analysis in upper Egypt
N2  - Rabbit hemorrhagic disease (RHD) is a contagious viral disease that threatens rabbit farms locally and globally. The disease causative agent is the 63-Rabbit_Haemorrhagic_Disease_Virus_Strains_Circulating_in_Rabbit_Population_RHD virus (RHDV) of the family Caliciviridae. The present study aimed to identify and characterize RHDV strains currently circulating in Upper Egypt provinces. A total of 20 suspected RHDV samples were collected from non-vaccinated rabbit flocks from January to December 2019 in Upper Egypt governorates (New Valley and Assuit), Egypt. The RHDV was confirmed through the hemagglutination test (HA) and reverse transcription-polymerase chain reaction (RT-PCR). Further characterization of selected 4 isolates was performed by nucleotide sequencing of a partial VP60 gene. All of 11 RHDV RT-PCR-positive samples were positive for HA activity against human RBCs type "O". Based on the nucleotide sequencing, the selected 4 isolates were clustered as RHDV-1 variant strains (G3-G5). The nucleotide sequence identities of the 4 isolates were 94.2-100 %, compared to available RHDV strains from GenBank. In conclusion, the presence of RHDV-1 variant strains was detected and confirmed that threatens the rabbit?s populations in New Valley and Assuit governorates.
PB  - Scienceline Publication, Ltd
JF  - Journal of World's Poultry Research
IS  - 3
ID  - eprints89
ER  -