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<http://eprints.science-line.com/id/eprint/89> <http://purl.org/dc/terms/title> "Isolation and Molecular Characterization of Rabbit Haemorrhagic Disease Virus Strains Circulating in Rabbit Population Using Sequencing and Phylogenetic Analysis in upper Egypt"^^<http://www.w3.org/2001/XMLSchema#string> .
<http://eprints.science-line.com/id/eprint/89> <http://purl.org/ontology/bibo/abstract> "Rabbit hemorrhagic disease (RHD) is a contagious viral disease that threatens rabbit farms locally and globally. The disease causative agent is the 63-Rabbit_Haemorrhagic_Disease_Virus_Strains_Circulating_in_Rabbit_Population_RHD virus (RHDV) of the family Caliciviridae. The present study aimed to identify and characterize RHDV strains currently circulating in Upper Egypt provinces. A total of 20 suspected RHDV samples were collected from non-vaccinated rabbit flocks from January to December 2019 in Upper Egypt governorates (New Valley and Assuit), Egypt. The RHDV was confirmed through the hemagglutination test (HA) and reverse transcription-polymerase chain reaction (RT-PCR). Further characterization of selected 4 isolates was performed by nucleotide sequencing of a partial VP60 gene. All of 11 RHDV RT-PCR-positive samples were positive for HA activity against human RBCs type \"O\". Based on the nucleotide sequencing, the selected 4 isolates were clustered as RHDV-1 variant strains (G3-G5). The nucleotide sequence identities of the 4 isolates were 94.2-100 %, compared to available RHDV strains from GenBank. In conclusion, the presence of RHDV-1 variant strains was detected and confirmed that threatens the rabbit’s populations in New Valley and Assuit governorates."^^<http://www.w3.org/2001/XMLSchema#string> .
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