@article{eprints89,
          author = {S. E. S. A. Abodalal and M. S. A. Hafez and E. A. E. M. Shosha and F. F. Warda and N. M. Hagag},
          volume = {11},
           title = {Isolation and Molecular Characterization of Rabbit Haemorrhagic Disease Virus Strains Circulating in Rabbit Population Using Sequencing and Phylogenetic Analysis in upper Egypt},
       publisher = {Scienceline Publication, Ltd},
          number = {3},
           pages = {302--311},
            year = {2021},
           month = {September},
         journal = {Journal of World's Poultry Research},
        keywords = {Upper Egypt, Nucleotide sequencing, Rabbit hemorrhagic disease virus, Reverse transcription-polymerase chain reaction, VP60},
             url = {http://eprints.science-line.com/id/eprint/89/},
        abstract = {Rabbit hemorrhagic disease (RHD) is a contagious viral disease that threatens rabbit farms locally and globally. The disease causative agent is the 63-Rabbit\_Haemorrhagic\_Disease\_Virus\_Strains\_Circulating\_in\_Rabbit\_Population\_RHD virus (RHDV) of the family Caliciviridae. The present study aimed to identify and characterize RHDV strains currently circulating in Upper Egypt provinces. A total of 20 suspected RHDV samples were collected from non-vaccinated rabbit flocks from January to December 2019 in Upper Egypt governorates (New Valley and Assuit), Egypt. The RHDV was confirmed through the hemagglutination test (HA) and reverse transcription-polymerase chain reaction (RT-PCR). Further characterization of selected 4 isolates was performed by nucleotide sequencing of a partial VP60 gene. All of 11 RHDV RT-PCR-positive samples were positive for HA activity against human RBCs type "O". Based on the nucleotide sequencing, the selected 4 isolates were clustered as RHDV-1 variant strains (G3-G5). The nucleotide sequence identities of the 4 isolates were 94.2-100 \%, compared to available RHDV strains from GenBank. In conclusion, the presence of RHDV-1 variant strains was detected and confirmed that threatens the rabbit?s populations in New Valley and Assuit governorates.}
}