eprintid: 845
rev_number: 9
eprint_status: archive
userid: 2
dir: disk0/00/00/08/45
datestamp: 2023-10-11 00:42:22
lastmod: 2023-10-11 00:42:22
status_changed: 2023-10-11 00:42:22
type: article
metadata_visibility: show
creators_name: Susalam, M.K.
creators_name: Harnentis, .
creators_name: Jamsari, .
creators_name: Marlida, Y.
title: NEW GROWTH MEDIUM FOR CULTURING LACTIC ACID BACTERIA AS PROBIOTIC CONSORTIUM ISOLATED FROM FERMENTED FISH (BUDU)
ispublished: pub
subjects: Q1
subjects: S1
subjects: SF
divisions: j11
full_text_status: public
keywords: Cell biomass; Fermentation; Medium pH; Probiotic consortium; Viability
note: Graduate Faculty of Animal Science, Universitas Andalas, Padang, 25163, Indonesia; Department of Nutrition and Feed Technology, Faculty of Animal Science, Universitas Andalas, Padang, 25163, Indonesia; Department of Genomic and Molecular Breeding, Universitas Andalas, Padang, 25163, Indonesia
abstract: This study aimed to obtain the best ratio of inoculums and types of alternative media in increasing the growth of the probiotic consortium with the observed variables consisting of viability, cell biomass and decrease in pH. Completely randomized design (CRD) factorial consisting of 2 factors with 3 replications, factor A were the probiotic consortium (A1: Lactobacillus parabuchneri: L. buchneri: L. harbinensis, Schieferilactobacillus harbinensis and Lentilactobacillus parabuchner) with ratio 1:1:1:1:1; A2: same consortium with ratio 1:1:1:1:2; A3: same consortium with ratio 1:1:1:2:1; A4: same consortium with ratio 1:1:2:1:1; A5: same consortium with ratio 1:2:1:1:1; A6: same consortium with ratio 2:1:1:1:1 and factor B were the type of alternative media (B1=control; B2=coconut water (90) + cassava flour (5) + fish waste flour (5); B3=tofu liquid waste (90) + flour onggok (5) + fish waste meal (5); B4= tofu whey (90) + onggok flour (5) + fish waste meal (5). The results showed that there was an interaction between factor A and factor B which was highly significant (P<0.01) on viability, cell biomass and decrease in medium pH. In conclusion, the best ratio of probiotic consortium was 1:1:1:2:1, with growth medium coconut water (90) + cassava flour (5) + fish waste flour (5) which resulted in a viability value of: 3, 02, cell biomass: 22.47 mg/ml and a decrease in the pH of the medium by 2.84. © 2023, Online Journal of Animal and Feed Research. All Rights Reserved.
date: 2023-05-27
publication: Online Journal of Animal and Feed Research
volume: 13
number: 3
publisher: Scienceline Publications, Ltd
pagerange: 199-203
id_number: 10.51227/ojafr.2023.30
refereed: TRUE
issn: 2228-7701
official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85161563038&doi=10.51227%2fojafr.2023.30&partnerID=40&md5=43d84370aee9679c8dce73ed0d1bcdb7
j_index: scopus
citation:    (2023)     NEW GROWTH MEDIUM FOR CULTURING LACTIC ACID BACTERIA AS PROBIOTIC CONSORTIUM ISOLATED FROM FERMENTED FISH (BUDU).   Online Journal of Animal and Feed Research.  pp. 199-203.  ISSN 2228-7701   
document_url: http://eprints.science-line.com/id/eprint/845/1/OJAFR%2013%283%29%20199-203%2C%202023.pdf