TY  - JOUR
SP  - 85
EP  - 94
AV  - public
Y1  - 2023/03/25/
SN  - 2322-4568
N2  - Besides its zoonotic importance, avian pathogenic Escherichia coli (APEC) causes substantial financial losses in the poultry industry globally. The progress of antimicrobial resistance in APEC is mainly associated with excessive antimicrobial use and improper sanitation. Since its beginning in the 1970s, the VITEK system has developed into the VITEK 2 system, which has used an automated system to perform all the steps required for microbial identification and antibiotic susceptibility rapidly and accurately. The present study aimed to update the available circulating data about APEC isolates by phenotypic identification, sero-grouping of APEC from broilers chickens and breeders in five governates of Egypt, investigation of their antibiotic resistance pattern by VITEK 2 system, and molecular identification of their virulence determinants. The prevalence of APEC isolated from the different internal organs (liver, lung, heart, heart blood, and spleen) was 67.5. The most prevalent serotypes were O125, 0114, O44, O127, O142, and O78. Virulence-associated genes (iutA, fimC, and papC) were detected at rates of 84.4, 74, and 54.8, respectively. The highest resistance was found against ampicillin (100), trimethoprim-sulfamethoxazole (80), and ampicillin-sulbactam (78.5), which indicates that the poultry farms need a surveillance and intervention system with proper accuracy and rapidity to prevent the misuse of antibiotics and APEC outbreaks © 2023, World's Veterinary Journal.All Rights Reserved.
A1  - Hamed, B.M.
A1  - Elenbaawy, M.I.
A1  - Mahmoud, H.
A1  - Ragab, E.
KW  - amikacin; ampicillin; cefazolin; cefepime; cefoxitin; ceftazidime; ceftriaxone; ciprofloxacin; cotrimoxazole; gentamicin; levofloxacin; meropenem; nitrofurantoin; RNA 16S; sultamicillin; tobramycin
KW  -  16srRNA gene; accuracy; animal tissue; antibiotic resistance; antibiotic sensitivity; Article; avian pathogenic Escherichia coli; bacterial gene; bacterial virulence; bacterium isolate; broiler; controlled study; Egypt; fimC gene; gene sequence; incubation time; iutA gene; microbial identification; nonhuman; papC gene; polymerase chain reaction
VL  - 13
JF  - World's Veterinary Journal
PB  - Scienceline Publication, Ltd
UR  - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85151852533&doi=10.54203%2fscil.2023.wvj9&partnerID=40&md5=40c8ad24b074ecd7367820338f34bc72
N1  - Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt
ID  - eprints821
IS  - 1
TI  - Investigation of Antibiotic Resistance Pattern and Virulence Determinants in Avian Pathogenic Escherichia Coli Isolated from Broiler Chickens in Egypt
ER  -