relation: http://eprints.science-line.com/id/eprint/806/
title: NETosis and Calcium influx in Dromedary Camel Neutrophils after In Vitro Toll-like Receptor Stimulation
creator: Albahrani, K.
creator: Alessa, J.
creator: Falemban, B.
creator: Alkuwayti, M.A.
creator: Hussen, J.
subject: SF Animal culture
description: Neutrophilic granulocytes are vital immune cells of the early response to pathogens. They contribute to the antimicrobial response through phagocytosis, production of reactive oxygen species, cytokine production, degranulation, and NET-formation. Neutrophil extracellular traps (NETs), also known as NETosis, are a critical antibacterial effector mechanism of cells of myeloid effector cells, including neutrophils and macrophages. Toll-like receptors (TLRs) are pattern recognition receptors (PRRs) that mediate pathogen sensing through the recognition of microbial structures known as pathogen-associated molecular patterns (PAMPs). The present study aimed to investigate the potential of several TLR ligands that mimic the sensing of bacterial and viral pathogens to stimulate NET-formation or Ca2+ influx in camel neutrophils. Neutrophils were purified from blood and were stimulated in vitro with ligands to TLR4, TLR2/1, TLR7/8, or TLR3. Net-formation was analyzed using the DNA-sensitive dye SYTOX� Green and staining with antibodies to the neutrophil's granular enzyme myeloperoxidase. Real-time stimulation-induced Ca2+ influx was measured using the Ca2+-sensitive dye Flou-4 and flow cytometry. Only the TLR4-ligand lipopolysaccharide (LPS) could induce NET-formation in camel neutrophils, while none of the investigated TLR agonists showed a Ca2+ influx-inducing effect in camel neutrophils. The current study represents the first report on the impact of direct activation of TLR on NET-formation and Ca2+ influx in camel neutrophils with a selective effect of LPS on NET-formation induction. Future studies may investigate the molecular mechanisms behind the different responsiveness of bovine and camel neutrophils to TLR stimulation. © 2023, World''s Veterinary Journal. All Rights Reserved.
publisher: Scienceline Publication, Ltd
date: 2023-06-25
type: Article
type: PeerReviewed
format: text
language: en
identifier: http://eprints.science-line.com/id/eprint/806/1/WVJ%2013%281%29%2C%20214-221%2C%20March%2025%2C%202023.pdf
identifier:    (2023)     NETosis and Calcium influx in Dromedary Camel Neutrophils after In Vitro Toll-like Receptor Stimulation.   World's Veterinary Journal.  pp. 214-221.  ISSN 2322-4568   
relation: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85158128278&doi=10.54203%2fSCIL.2023.WVJ23&partnerID=40&md5=0cab30712022e412f580611f58c65d04
relation: 10.54203/SCIL.2023.WVJ23