eprintid: 80
rev_number: 8
eprint_status: archive
userid: 3
dir: disk0/00/00/00/80
datestamp: 2022-05-27 17:27:00
lastmod: 2022-05-27 17:27:00
status_changed: 2022-05-27 17:27:00
type: article
metadata_visibility: show
creators_name: A.shedeed, E.
creators_name: D. El-Hariri, M.
creators_name: A.Nasef, S.
creators_name: Jakee, J. E.
creators_id: jeljakee@yahoo.com
title: Immunological Study on Salmonellae Isolated from Different Sources
ispublished: pub
subjects: Q1
subjects: SF
divisions: j10
full_text_status: public
keywords: Outer Membrane Proteins, Salmonella, SDS-PAGE, Western blotting
abstract: Salmonella infection is a critical veterinary and medical problem worldwide and is a major issue in the food industry. Non-typhoidal Salmonella is known as an important pathogen causing gastroenteritis. The Outer Membrane Proteins (OMPs) of Gram negative bacteria are significant for virulence, host immune responses and drug therapy targets. Enhanced diagnosis of live poultry colonized with Salmonella species is required to avoid foodborne diseases. The present study was based on molecular characterization of OMPs among four Salmonella serovars (S. Typhimurium, S. Enteritidis, S. Kentucky and S. Anatum) using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The OMPs profiling showed more than 70 protein bands ranged in size from 208 kDa to below 16 kDa which were detected using Total Lab 1D 12.2 software. All Salmonella strains had a band at 54-60 kDa, 45-53 kDa, 36-39 kDa and 26-31 kDa. Eleven strains exhibited a band at 41-46 kDa and 33-35 kDa. Nine strains had a band at 61-69 kDa. Eight strains exhibited a band at 135-145 kDa and 72-79 kDa. Seven strains had a band at 108-123 kDa and 83-91 kDa. In the Western blot analysis, the prepared hyperimmune anti serum of each Salmonella serovars reacted with the 35 kDa protein band. It is concluded that the identification of novel immunogenic proteins would be useful in developing ELISA-based diagnostic assays with a higher specificity.
date: 2020-06-14
publication: Journal of World's Poultry Research
volume: 10
number: 2s
publisher: Scienceline Publication, Ltd
pagerange: 285-291
id_number: 10.36380/JWPR.2020.34
refereed: TRUE
issn: 2322455X (ISSN)
official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85099936504&doi=10.36380%2fJWPR.2020.34&partnerID=40&md5=5aafc009b84991e825c6dd1af83b7070
j_index: scopus
citation:    (2020)     Immunological Study on Salmonellae Isolated from Different Sources.   Journal of World's Poultry Research.  pp. 285-291.  ISSN 2322455X (ISSN)   
document_url: http://eprints.science-line.com/id/eprint/80/1/JWPR%2010%282S%29%20285-291%2C%202020.pdf