eprintid: 786
rev_number: 10
eprint_status: archive
userid: 5
dir: disk0/00/00/07/86
datestamp: 2023-10-17 15:40:57
lastmod: 2023-10-17 15:40:57
status_changed: 2023-10-17 15:40:57
type: article
metadata_visibility: show
creators_name: Mekuria, T.A.
creators_name: Kinde, M.Z.
title: Basic Principles and Applications of Live Cell Microscopy Techniques: A Review
ispublished: pub
subjects: SF
divisions: j13
full_text_status: public
keywords: fluorescent dye, Article; biomedicine; confocal laser scanning microscopy; darkfield microscopy; differential interference contrast microscopy; economic aspect; financial viability; fluorescence; fluorescence microscopy; human; illumination; image quality; imaging; intermethod comparison; job experience; laser microscopy; live cell imaging; live cell microscopy; microscopy; multiphoton confocal laser scanning microscopy; nonhuman; phase contrast microscopy; research; spinning disk confocal microscopy; stimulated emission depletion microscopy; super resolution microscopy; transmitted light microscopy; wide field fluorescence microscopy
note: Department of Veterinary Science, College of Agriculture and Natural Resource, Assosa University, Ethiopia; Department of Veterinary Biomedical Sciences, College of Veterinary Medicine and Animal Sciences, University of Gondar, Ethiopia
abstract: Live cell imaging has provided great benefits in studying multiple processes and molecular interactions within and/or between cells. This review aimed to describe the common live cell microscopy techniques and briefly explain their principles and applications. A wide range of microscopic techniques, from conventional transmitted light to an array of fluorescence microscopy techniques, including advanced super-resolution techniques, can be applied for live-cell imaging. Transmitted light microscopy uses focused transmitted light that goes through a condenser to achieve a very high illumination on the specimen. On the other hand, fluorescence microscopy uses reflected light to capture images of cells or molecules that have been fluorescently dyed. Techniques for transmitted light microscopy are simple to use but have poor resolution. Although the resolution of fluorescent microscopy techniques is only approximately 200-300 nm, this is nevertheless an improvement over conventional transmitted methods. Conventional light microscopy�s resolution was improved by the introduction of the super-resolution microscopy technology family. These methods �break� the diffraction limit, enabling fluorescence imaging with resolutions up to ten times higher than those possible with traditional methods. Each live cell imaging method has advantages and drawbacks. The primary deciding criteria for choosing the type of microscope are the study�s objectives, previous experience, the researcher�s interests, and financial viability. Hence, a thorough understanding of the technique and application of the various live-cell microscopy methods is paramount in life science studies. © 2022,World''s Veterinary Journal.All Rights Reserved.
date: 2022-12-25
publication: World's Veterinary Journal
volume: 12
number: 3
publisher: Scienceline Publication, Ltd
pagerange: 339-346
id_number: 10.54203/SCIL.2022.WVJ43
refereed: TRUE
issn: 2322-4568
official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85145925067&doi=10.54203%2fSCIL.2022.WVJ43&partnerID=40&md5=7dd435c9ee9252e8029dcc7049accc83
j_index: scopus
citation:    (2022)     Basic Principles and Applications of Live Cell Microscopy Techniques: A Review.   World's Veterinary Journal.  pp. 339-346.  ISSN 2322-4568   
document_url: http://eprints.science-line.com/id/eprint/786/1/article/74/WVJ%2012%283%29%2C%20339-346%2C%20September%2025%2C%202022.pdf