eprintid: 785 rev_number: 10 eprint_status: archive userid: 5 dir: disk0/00/00/07/85 datestamp: 2023-10-17 15:40:14 lastmod: 2023-10-17 15:40:14 status_changed: 2023-10-17 15:40:14 type: article metadata_visibility: show creators_name: Mohamed, S.N. creators_name: Mohamed, H.A. creators_name: Elbarbary, H.A. creators_name: EL-Roos, N.A. title: Antimicrobial Effects of Selenium and Chitosan Nanoparticles on Raw Milk and Kareish Cheese ispublished: pub subjects: SF divisions: j13 full_text_status: public keywords: chitosan nanoparticle; selenium nanoparticle, antimicrobial activity; Article; bacterial count; cheese; concentration (parameter); controlled study; cryopreservation; Enterobacteriaceae; food contamination; food storage; microorganism; mold; particle size; raw milk; refrigeration; Staphylococcus; temperature measurement note: Food Hygiene Animal Health Research Institute, Egypt; Food Hygiene and Control Department, Faculty of Veterinary Medicine, Benha University, Egypt abstract: The contamination of milk and its dairy products with different microorganisms could cause public health hazards. Antibacterial nanoparticles (NPs) are a novel way to ensure that milk and milk products are safe. The present study investigated the effect of chitosan NPs (CS-NPs) and selenium NPs (Se-NPs) on some microorganisms, which consequently affect raw milk and Kareish cheese. Small-sized nanomaterials of Se-NPs and CS-NPs at the size of approximately 20 nm were used in this study. The samples were 700 ml raw milk and 700g Kareish cheese manufactured from 3000 mg milk. The concentrations of used nanoparticles were 0.5, 1, and 1.5 for Se-NPs and 2.5, 5, and 10 for CS-NPs. They were used to improve the microbial properties of milk and Kareish cheese samples during storage at the refrigerated temperature of 4°C. The aerobic plate count, Enterobacteriaceae count, Staphylococcus count, and mold count were significantly reduced in milk and Kareish cheese samples treated with CS-NPs and Se-NPs. The study has confirmed that CS-NPs and Se-NPs indicated high antimicrobial activity against the studied microorganisms at all concentrations although CS-NPs were more effective than Se-NPs. It can be concluded that these NPs can be used as preservatives in milk and milk products, such as Kareish cheese. In addition, increasing the concentrations of these NPs by 10 for CS-NPS and 1.5 for Se-NPS boosted their effects. © 2022, World''s Veterinary Journal. All Rights Reserved. date: 2022-12-25 publication: World's Veterinary Journal volume: 12 number: 3 publisher: Scienceline Publication, Ltd pagerange: 330-338 id_number: 10.54203/SCIL.2022.WVJ42 refereed: TRUE issn: 2322-4568 official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85145933576&doi=10.54203%2fSCIL.2022.WVJ42&partnerID=40&md5=4de07675bbced2acc3fd3c8c8e4edc6c j_index: scopus citation: (2022) Antimicrobial Effects of Selenium and Chitosan Nanoparticles on Raw Milk and Kareish Cheese. World's Veterinary Journal. pp. 330-338. ISSN 2322-4568 document_url: http://eprints.science-line.com/id/eprint/785/1/article/74/WVJ%2012%283%29%2C%20330-338%2C%20September%2025%2C%202022.pdf