eprintid: 781
rev_number: 10
eprint_status: archive
userid: 5
dir: disk0/00/00/07/81
datestamp: 2023-10-17 15:37:09
lastmod: 2023-10-17 15:37:09
status_changed: 2023-10-17 15:37:09
type: article
metadata_visibility: show
creators_name: Samy, A.A.
creators_name: Arafa, A.A.
creators_name: Hedia, R.H.
creators_name: Ibrahim, E.S.
title: Multiple Drug Resistance Salmonella and Antibiotic Residues in Egyptian Animal Products
ispublished: pub
subjects: SF
divisions: j13
full_text_status: public
keywords: amoxicillin; ampicillin; antibiotic agent; cefradine; ciprofloxacin; drug residue; erythromycin; oxytetracycline; streptomycin, animal product; antibiotic resistome; antibiotic sensitivity; Article; bacterial gene; bacterial strain; bacterium isolate; bacterium isolation; beef; bone marrow; column chromatography; consumer; controlled study; dairy product; dairying; disk diffusion; drug determination; drug milk level; Egypt; health hazard; high performance liquid chromatography; incidence; intestine tissue; liver tissue; meat; multidrug resistance; nonhuman; poultry product; randomization; residue analysis; reversed phase high performance liquid chromatography; Salmonella; veterinary medicine
note: Department of Microbiology and Immunology, National Research Centre, Giza, Egypt
abstract: Food of animal origin is considered a primary source of foodborne diseases. The misuse of antibiotics to treat and control many bacterial diseases in farm animals has led to multiple antibiotic-resistant pathogens in contaminated food that can seriously threaten public health. The present study aimed to highlight the impact of antimicrobial misuse in Egyptian beef meat, poultry, and dairy farms on the emergence of multiple antibiotic resistance Salmonella and the detection of antibiotic residues in milk. A total of 1050 samples were collected randomly from poultry (liver, intestinal content, and bone marrow), meat, and milk products from different Egyptian governorates. Salmonellae were isolated from the collected samples and subjected to antimicrobial sensitivity testing through disk diffusion test using the most commonly used seven antibiotics in veterinary fields (cefradine, ciprofloxacin, oxytetracycline, erythromycin, amoxicillin, ampicillin, and streptomycin). The detection of oxytetracycline residue in milk samples was performed by high-performance liquid chromatography (HPLC). Most isolated Salmonellae were multiple drug resistant with an incidence rate of 8.6, 15.4, and 4 from poultry, meat-associated products, and milk-associated products, respectively, from different governorates. Antibiogram test showed that the isolated Salmonella from poultry, meat, and milk samples were resistant to oxytetracycline at 100, 31.4, and 43, to amoxicillin at 73.3, 31, and 50, and to ampicillin 66.6, 50, and 57, respectively. No resistance to ciprofloxacin was detected in Salmonella isolates from all samples. Using HPLC, oxytetracycline residues were detected in milk samples. In conclusion, more attention should be paid to the connection between the widespread emergence of antibiotic-resistant Salmonella in Egypt and the overuse of antimicrobials in poultry, dairy, and meat farms. This connection affects consumer health and increases the likelihood of resistance genes spreading between different bacterial species © 2022, World''s Veterinary Journal. All Rights Reserved.
date: 2022-12-25
publication: World's Veterinary Journal
volume: 12
number: 4
publisher: Scienceline Publication, Ltd
pagerange: 363-373
id_number: 10.54203/scil.2022.wvj46
refereed: TRUE
issn: 2322-4568
official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85145833097&doi=10.54203%2fscil.2022.wvj46&partnerID=40&md5=f2f63eab02cc7a61087f1c9eeac9e9e7
j_index: scopus
citation:    (2022)     Multiple Drug Resistance Salmonella and Antibiotic Residues in Egyptian Animal Products.   World's Veterinary Journal.  pp. 363-373.  ISSN 2322-4568   
document_url: http://eprints.science-line.com/id/eprint/781/1/article/74/WVJ%2012%284%29%2C%20363-373%2C%20December%2025%2C%202022.pdf