eprintid: 780
rev_number: 10
eprint_status: archive
userid: 5
dir: disk0/00/00/07/80
datestamp: 2023-10-17 15:36:32
lastmod: 2023-10-17 15:36:32
status_changed: 2023-10-17 15:36:32
type: article
metadata_visibility: show
creators_name: Kukhtyn, M.
creators_name: Malimon, Z.
creators_name: Salata, V.
creators_name: Rogalskyy, I.
creators_name: Gutyj, B.
creators_name: Kladnytska, L.
creators_name: Kravcheniuk, K.
creators_name: Horiuk, Y.
title: The Effects of Antimicrobial Residues on Microbiological Content and the Antibiotic Resistance in Frozen Fish
ispublished: pub
subjects: SF
divisions: j13
full_text_status: public
keywords: aminoglycoside antibiotic agent; amoxicillin; ampicillin; antiinfective agent; apramycin; biocide; cefalexin; chlortetracycline; ciprofloxacin; danofloxacin; difloxacin; dihydrostreptomycin; doxycycline; enrofloxacin; flumequine; gentamicin; josamycin; kanamycin; lincomycin; marbofloxacin; nafcillin; nalidixic acid; nordifloxacin; norfloxacin; ostetracycline; oxacillin; oxalic acid; paromomycin; penicillin derivative; penicillin G; penicillin V; spectinomycin; spiramycin; streptomycin; sulfabenzamide; sulfacetamide; sulfadiazine; sulfadimethoxine; sulfadimidine; sulfadoxine; sulfaguanidine; sulfamerazine; sulfamethizole; sulfamethoxazole; sulfamethoxypyridazine; sulfamonomethoxine; sulfaphenazole; sulfapyridine; sulfathiazole; sulfinoxolin; tetracycline; tiamulin; trimethoprim; unclassified drug, Acinetobacter; Aeromonas; Alcaligenes; antibacterial residue; antibiotic resistance; antibiotic sensitivity; antimicrobial residue; Article; bacterium isolate; capelin; China; controlled study; dorado; Enterobacter; European Union; fillet (fish); fish; flounder; food contamination; frozen fish; frozen food; gene; gene expression; herring; indicator organism; Italy; lackerda; mackerel; microbial contamination; microorganism; New Zealand; nonhuman; Norway; pink salmon; Pollachius virens; Pseudomonas; quantitative analysis; Russian Federation; salka; salmonine; sampling; sea food; Ukraine; United Kingdom; United States; Viet Nam
note: Ternopil Ivan Pului National Technical University, Ruska,56, Ternopil, 46001, Ukraine; State Research Institute for Laboratory Diagnostics and Veterinary and Sanitary Expertise, Donetska,30, Kyiv, 02000, Ukraine; Lviv National University of Veterinary Medicine and Biotechnologies named after S. Z. Gzhytskyj, Pekarska,50, Lviv, 79010, Ukraine; Main Department of the State Food and Consumer Service in Ternopil region, Mykulynetska,20, Ternopil, 46002, Ukraine; Academician M.F.Gulyi National University of Life and Environmental Sciences of Ukraine, Heroyiv Oborony st.,15, Kyiv, 03041, Ukraine; Higher educational institution «Podillia State University», Schevchenko,13, Kamianets-Podilskyi, 32301, Ukraine
abstract: As fish are perishable foods, their storage conditions require appropriate sanitary and temperature regimes. The producers commonly use various antibiotics to stop fish�s microbiological and biochemical processes. The current research aimed to examine antibacterial residues in frozen fish (Argentina, flounder, lackerda, mackerel, capelin, salka, saithe, herring, dorado, and pink salmon) to find their influence on the quantitative content of microorganisms and to determine the sensitivity of isolated psychrotrophic bacteria to antibiotics. A total of 75 samples were collected from the fillets of frozen fish species. These fish were imported from Norway (16 samples), Vietnam (24 samples), Russian Federation (8 samples), China (14 samples), New Zealand (2 samples), Italy (2 samples), United States (4 samples), and United Kingdom (5 samples). The obtained results revealed that aminoglycosides (Gentamicin, Kanamycin, Spectinomycin, Dihydrostreptomycin, Paromomycin, and Apramycin) were in 45.6 ± 1.4 of frozen fish. The findings indicated the presence of some antibacterial residues (Nalidixic acid, antibiotics: Apramycin, Kanamycin, Tiamulin, and Nafcillin) in frozen fish, the definition of which has not been specified in the EU Regulation. This gives grounds to prohibit the use or develop standards for the maximum permissible concentration of these antibacterial substances in fish. The most common psychrotrophic bacteria isolated from frozen fish without antibacterial residues were highly sensitive to antibiotics, including Penicillin, Tetracycline groups, and Aminoglycosides. Therefore, it can be concluded that the residual levels of various biocides found in fish are a source for the expression of multi-resistance genes, which can be transmitted to consumers in the food chain © 2022, World''s Veterinary Journal. All Rights Reserved.
date: 2022-12-25
publication: World's Veterinary Journal
volume: 12
number: 4
publisher: Scienceline Publication, Ltd
pagerange: 374-381
id_number: 10.54203/scil.2022.wvj47
refereed: TRUE
issn: 2322-4568
official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85145837341&doi=10.54203%2fscil.2022.wvj47&partnerID=40&md5=85bee128bcbe56feb2bee1fca84641fe
j_index: scopus
citation:    (2022)     The Effects of Antimicrobial Residues on Microbiological Content and the Antibiotic Resistance in Frozen Fish.   World's Veterinary Journal.  pp. 374-381.  ISSN 2322-4568   
document_url: http://eprints.science-line.com/id/eprint/780/1/article/74/WVJ%2012%284%29%2C%20374-381%2C%20December%2025%2C%202022.pdf