TY - JOUR SN - 2322-455X A1 - Firmansyah, G.I. A1 - Perdamaian, A.B.I. A1 - Daryono, B.S. TI - Phenotypic Characters and TYRP1 Polymorphism of F4 Golden Kamper Hybrid Chickens (Gallus gallus domesticus Linnaeus, 1758) UR - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85134007704&doi=10.36380%2fjwpr.2022.12&partnerID=40&md5=94aaa40ab86308603cfc795dc066321a N1 - Laboratory of Genetics and Breeding, Faculty of Biology, University of Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Sleman Yogyakarta, Indonesia Y1 - 2022/06/25/ AV - public EP - 116 JF - Journal of World's Poultry Research VL - 12 N2 - Golden Kamper is a local meat-typed chicken with four generations of Pelung male and Layer female selective breeding. This chicken has various plumage colors and patterns. Therefore, the desired plumage color is red barred plumage (B1). In chickens, the missense mutation in the Tyrosinase-related-proteins 1 (TYRP1) causes a chocolate color plumage (choc) with an epistatic effect on barred plumage. The current study aimed to observe the growth of 16 chickens from hatching until 49 days of age to investigate the phenotypic characteristics, especially plumage color at 49-day-old chickens, then to determine the effect of the TYRP1 polymorphism on F4 Golden Kamper phenotypes. The methods used in this study included selective breeding among F3 Golden Kamper, collection of F3 Golden Kamper?s eggs, then rearing the day-old chickens of F4 Golden Kamper. Phenotypic data were collected and blood collection was performed for DNA isolation, DNA amplification, and sequencing. Of 16 F4 Golden Kamper, all chickens had a uniform comb type of single (rprp, 100). The produced shank colors were white (31.25), yellow (62.5), and blackish gray (6.25). The plumage colors were red barred (12.5), white barred (12.5), brown (68.75), and chocolate (6.25). The bodyweight of F4 Golden Kamper at the age of 7 weeks reached 597.3 g. The morphometric results indicated that F4 Golden Kamper had the same posture and body proportions as Pelung chickens, however, with a higher weight. Fourteen substitutions were found in the TYRP1 fragment of F4 Golden Kamper. The single nucleotide polymorphisms (SNP) had no correlation with the chocolate plumage phenotype in F4 Golden Kamper. The evaluated SNPs in TYRP1 were not associated with the brown plumage color phenotype. © 2022 PB - Scienceline Publication, Ltd SP - 107 ID - eprints702 KW - Chicken; Golden Kamper; Phenotype; Polymorphism; TYRP1 IS - 2 ER -