TY  - JOUR
TI  - Heterogeneity of Yersinia ruckeri Isolated from Rainbow Trout (Oncorhynchus mykiss) Infected with Enteric Red Mouth Disease
SP  - 19
SN  - 2322-4568
EP  - 27
N1  - Department of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Iran; Liver and Digestive Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran; Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran
KW  - ampicillin; cefalotin; chloramphenicol; ciprofloxacin; enrofloxacin; gentamicin; lincomycin; lincomycin plus spectinomycin; oxytetracycline; RNA 16S; sulfamerazine plus trimethoprim
KW  -  antibiotic resistance; antibiotic sensitivity; Article; bacterium isolate; dispersity; enteric redmouth disease; enterobacterial repetitive intergenic consensus polymerase chain reaction; Escherichia coli; gel electrophoresis; molecular typing; nonhuman; Oncorhynchus mykiss; phylogenetic tree; random amplified polymorphic DNA polymerase chain reaction; repetitive extragenic palindromic polymerase chain reaction; Simpson index; Yersinia ruckeri; yersiniosis
AV  - public
A1  - Aflakian, F.
A1  - Nikoueian, H.
A1  - Salimizand, H.
A1  - Rad, M.
A1  - Zomorodi, A.R.
IS  - 1
PB  - Scienceline Publication, Ltd
JF  - World's Veterinary Journal
VL  - 12
Y1  - 2022/03/25/
ID  - eprints690
N2  - Regarding the increasing prevalence of antimicrobial resistance as a global menace, typing procedures are of great importance in epidemiological surveys. In the current study, interspecies differences of Yersinia ruckeri (Y. ruckeri) isolates were detected by antimicrobial susceptibility profiling and molecular traits to determine the relationship between isolates. The current study was conducted on 27 Y. ruckeri isolates collected from 40 infected rainbow trout (Oncorhynchus mykiss) with clinical enteric red mouth disease in Mashhad, Iran. Interspecies differences of all isolates were detected by antimicrobial susceptibility profiling using disk diffusion method, Repetitive Extragenic Palindromic PCR (REP-PCR), Enterobacterial Repetitive Intergenic Consensus PCR (ERIC-PCR), and Random Amplification of Polymorphic DNA PCR (RAPD-PCR) to determine the relationship between isolates. Simpson's diversity index was calculated for each typing technique. The most phenotype resistant was against ampicillin, chloramphenicol, and lincomycin. Furthermore, enrofloxacin and ciprofloxacin showed the highest activity (100) against isolates. According to the results, the highest diversity index was observed in the REP-PCR method (D = 0.91). In conclusion, REP-PCR can be a powerful technique for epidemiological studies. © 2022. All Rights Reserved.
UR  - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85128309029&doi=10.54203%2fscil.2022.wvj3&partnerID=40&md5=c5f80cb8c6f40823e851311be88e6713
ER  -