eprintid: 658
rev_number: 8
eprint_status: archive
userid: 5
dir: disk0/00/00/06/58
datestamp: 2022-12-03 01:14:23
lastmod: 2022-12-03 01:14:23
status_changed: 2022-12-03 01:14:23
type: article
metadata_visibility: show
creators_name: El Akkad, D.M.H.
creators_name: Ramadan, R.M.
creators_name: Auda, H.M.
creators_name: El-Hafez, Y.N.A.
creators_name: El-Bahy, M.M.
creators_name: Abdel-Radi, S.
title: Improved Dot-ELISA Assay Using Purified Sheep Coenurus cerebralis Antigenic Fractions for the Diagnosis of Zoonotic Coenurosis
ispublished: pub
subjects: Q1
subjects: SF
divisions: j13
full_text_status: public
keywords: Antigen; Coenurus cerebralis; Dot-elisa; Human; Scolices; Sheep
note: Department of Parasitology, Faculty of Medicine, Cairo University, Giza, Egypt; Department of Parasitology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt; Department of Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt; Department of Parasitology, Faculty of Medicine, Fayoum University, Egypt
abstract: Clinicians face significant problems in the diagnosis of zoonotic coenurosis. The current study aimed to develop an improved dot-Enzyme-linked-immunosorbent assay (dot-ELISA) for the diagnosis of zoonotic coenurosis using sheep Coenurus cerebralis scolices purified antigen (CcS-Ag) and to compare the obtained results with those of indirect ELISA and Enzyme-linked immunoelectrotransfer blot technique (EITB). Sera were collected from humans and sheep infected or suspected of infection with Coenurus cerebralis, control cases, and cases infected with other parasites. The CcS-Ag was proved to be the most specific antigen. This antigen was fractionated, and its specific polypeptides against anti-C. cerebralis antibodies (ACc-Ab) were identified using EITB. Fractions at the molecular weight (MW) of 48 and 58 kDa were proved as the only specific ones, eluted from the gel and concentrated, then dotted on the NC sheet as pooled antigen before its evaluation in the diagnosis of infection using dot-ELISA. Dot-ELISA demonstrated absolute 100 sensitivity and 100 specificity as recorded by EITB, compared to both fractions on a nitrocellulose (NC) sheet using surgically proved infected human or sheep sera as a gold standard. Diagnosis by ELISA using crude CcS-Ag revealed similar sensitivity but lower specificity (75). The diagnostic accuracy of dot-ELISA was proved by comparing its results with postmortem data obtained post slaughtering of 20 suspected sheep and patients investigated by computed tomography (CT) and magnetic resonance imaging (MRI). In conclusion, the selection of specific fractions after EITB to be used in dot-ELISA improved the diagnostic value of the test as a diagnostic tool gathering the benefits of ELISA and EITB. © Nikkhah A and Alimirzaei M (2022). Colibacillosis and Colisepeticemia in Newborn Calves: Towards Pragmatic Treatment and Prevention. World Vet. J., 12 (3): 230-236. DOI: https://dx.doi.org/10.54203/scil.2022.wvj29
date: 2022-09-25
publication: World's Veterinary Journal
volume: 12
number: 3
publisher: Scienceline Publication, Ltd
pagerange: 237-249
id_number: 10.54203/scil.2022.wvj30
refereed: TRUE
issn: 2322-4568
official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85138826163&doi=10.54203%2fscil.2022.wvj30&partnerID=40&md5=dd38308cdeeca7dfcc608d5be02b42f0
j_index: scopus
citation:    (2022)     Improved Dot-ELISA Assay Using Purified Sheep Coenurus cerebralis Antigenic Fractions for the Diagnosis of Zoonotic Coenurosis.   World's Veterinary Journal.  pp. 237-249.  ISSN 2322-4568   
document_url: http://eprints.science-line.com/id/eprint/658/1/WVJ%2012%283%29%2C%20237-249%2C%20September%2025%2C%202022.pdf