%L eprints621
%D 2021
%R 10.51227/OJAFR.2021.30
%J Online Journal of Animal and Feed Research
%V 11
%I Scienceline Publication
%P 206-212
%T QUALITY CHANGES IN SPERMATOZOA OF EXOTIC MUZAFFARNAGARI CROSS-BREED RAM SEMEN DURING THE STAGES OF FROZEN PRODUCTION
%X Good quality frozen semen is a prerequisite for successful artificial insemination. Changes in the
quality of sperm structure incur during semen processing of cryo-production. The current study, is, therefore,
designed to evaluate the Muzaffarnagari cross-breed ram sperm at the fresh diluted stage, pre-freeze, and
after freezing stages of frozen production. The semen collected was enlarged to include the A-part of 37°C
egg yolk media. After cooling to 5ºC for 2h, B-part of tris-citric acid egg yolk glycerol media added with Apart
and equilibrated at 5ºC for further 2h, transferred to 0.25 mL straws, placed in nitrogen vapor, frozen and
thawed and then analyzed. Sperm samples were assessed in fresh, pre-freezing, and post Thawing phases for
sperm motility, live sperm, ordinary sperm morphology, and intact sperm membrane. The mean percentage
of sperm motility at fresh diluted stage (81.67±0.93%) was decreased significantly (p<0.05) pre-freezing
stage (77.33±0.83%) because of refreshment and balance, and after freezing and thawing (44±2.45 percent)
decreased further (p<0.05). The Mean percentage of live sperm was higher (p<0.05) at fresh diluted stage
(91.27±0.57%) compared to pre-freeze (73.67±0.86%) and freezing stage (48.1±0.76%). The morphology
normal sperm reduced significantly (p<0.05) from fresh diluted stage (85.27±0.64%) to pre-freezing stage
(83.13±0.74). Their difference between the pre-freezing and the freezing stage is not significant (p>0.05)
(80.2±1.28 percent). The mean intact sperm membrane percentage decreased significantly (p<0.05) from
freshly diluted (82.4±0.77%) to pre-freezing (69.73±92%) and post-freezing (56.47±1.15%), respectively. The
percentage of intact acrosomes of spermatozoa also significantly (p<0.05) decreased from fresh stage
(95.8±0.39%) to pre-freeze (91.27±0.37%) and freezing stage (77.4±0.81%). In conclusion, during cryo-
freezing periods the sperm showed the highest motility, live and membrane damage.
%A M. Asaduzzaman
%A A. Saha
%A S. Akter
%A S. Biswas
%A M. G. S. Alam
%A F. Y. Bari
%K Frozen production, Muzaffarnagari cross-breed ram, Quality changes.
%N 6