TY  - JOUR
TI  - THE SPREADING AND MOLECULAR CHARACTERIZATION OF THE WATER MOULD Saprolegnia parasitica IN AL-DIWANIYAH RIVER OF IRAQ
SP  - 194
SN  - 22287701 (ISSN)
EP  - 199
KW  - Aquatic fungi
KW  -  Fish
KW  -  Fungal disease
KW  -  Molecular characterization
KW  -  Saprolegnia parasitica
AV  - public
A1  - Al-Zamili, M. M. A.
A1  - Aboud, M. K.
IS  - 4
PB  - Scienceline Publication
JF  - Online Journal of Animal and Feed Research
VL  - 12
Y1  - 2022/07/30/
ID  - eprints619
N2  - The water mold Saprolegnia parasitica is a chief species of oomycetes that affects a wide
range of plant, natural ecosystems, fish and the aquaculture industry. The current study aimed to
investigate the isolation and identification of some aquatic fungi like Saprolegnia spp. and also molecular
characterization of Saprolegnia parasitica in the water of Al-Diwaniyah river of Iraq using the baiting
method for isolation and PCR polymerase chain reaction for molecular diagnosis of fungi. A total of 60
samples were taken from three study sites of river: 25 samples of Al-Shafi?iah city bridge (Najaf road) as
first site (S1); 25 samples of Hawly Al-Jamiah road bridge, Umm Al-Khail area, as second site (S2); and 10
samples of Al-Orouba bridge as the third site (S3). Molecular diagnosis was carried out by PCR examination
using primers for the rDNA gene and its presence in Saprolegnia parasitica, as DNA was obtained at a
concentration of 685.4-99.4 ?g/?l and a purity of 1.92-1.8. The samples from the first site gave the
highest number of 18 isolates (45%), followed by samples from the second site with 14 isolates (35%). The
samples from the third site showed a number of 10 isolates (20%). The species S. parasitica was more
visible during February 2020 with a number of 17 isolates, and less visible in April 2021, with a number of
3 isolates. In conclusion, the variation in the presence of the types of infectious aquatic fungi
Saprolegnia spp. in the Al-Diwaniyah river of Iraq in different sites and months of 2021 showed a
high percentage of isolates for Al-Shafi?iah city bridge (Najaf road) site and the highest number of
Saprolegnia parasitica in February 2020. There is a need for further examination of oomycetes in
different sites of rivers of Iraq in different months.
UR  - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85135826435&doi=10.51227%2fojafr.2022.26&partnerID=40&md5=e64222ff7b28d6abdf6cc0515b0dd2f1
ER  -