eprintid: 538 rev_number: 10 eprint_status: archive userid: 5 dir: disk0/00/00/05/38 datestamp: 2022-05-04 01:24:59 lastmod: 2022-05-04 23:48:03 status_changed: 2022-05-04 01:24:59 type: article metadata_visibility: show creators_name: Untari, T. creators_name: Kristianingrum, Y. P. creators_name: Kusumawati, A. creators_id: yuli_purwandari@mail.ugm.ac.id title: Detection of Bovine Herpesvirus 1 from Semen by Real-time PCR to Prevent the Spread of Infectious Bovine Rhinotracheitis Infection ispublished: pub subjects: Q1 subjects: SF divisions: j13 full_text_status: public keywords: Artificial insemination, Bovine Herpesvirus 1, Infectious Bovine Rhinotracheitis, Real-time PCR, Semen abstract: Infectious Bovine Rhinotracheitis (IBR) can be transmitted by livestock seeds and semen, through the process of artificial insemination. Therefore, it is necessary to detect the presence of the Bovine Herpesvirus 1 (BHV1) in semen through artificial insemination and breeding centers of Indonesia. The current study aimed to detect the presence of the virus in semen as a source of IBR disease transmission in Indonesia. A total of 27 semen samples from artificial insemination and breeding centers (Sembawa, Lembang, Ungaran, and Sleman) in Indonesia have been examined and identified using the real-time PCR (qPCR) technique. The result showed that all samples were negative to BHV1. This indicated that semen from Ungaran, Sembawa, Sleman, and Lembang was safe to be used as a semen source for artificial insemination. date: 2021-12-25 publication: World's Veterinary Journal volume: 11 number: 4 publisher: Scienceline Publication, Ltd pagerange: 709-712 id_number: https://dx.doi.org/10.54203/scil.2021.wvj89 refereed: TRUE issn: 23224568 (ISSN) official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85123265800&doi=10.54203%2fscil.2021.wvj89&partnerID=40&md5=3d4921565277e16e1f19eaac3e85618f j_index: scopus citation: (2021) Detection of Bovine Herpesvirus 1 from Semen by Real-time PCR to Prevent the Spread of Infectious Bovine Rhinotracheitis Infection. World's Veterinary Journal. pp. 709-712. ISSN 23224568 (ISSN) document_url: http://eprints.science-line.com/id/eprint/538/1/WVJ%2011%284%29%20709-712%2C%20December%2025%2C%202021.pdf