%J World's Veterinary Journal
%I Scienceline Publication, Ltd
%T Detection of Bovine Herpesvirus 1 from Semen by Real-time PCR to Prevent the Spread of Infectious Bovine Rhinotracheitis Infection
%K Artificial insemination, Bovine Herpesvirus 1, Infectious Bovine Rhinotracheitis, Real-time PCR,  Semen
%P 709-712
%A T. Untari
%A Y. P. Kristianingrum
%A A. Kusumawati
%V 11
%D 2021
%X Infectious Bovine Rhinotracheitis (IBR) can be transmitted by livestock seeds and semen, through the process of artificial insemination. Therefore, it is necessary to detect the presence of the Bovine Herpesvirus 1 (BHV1) in semen through artificial insemination and breeding centers of Indonesia. The current study aimed to detect the presence of the virus in semen as a source of IBR disease transmission in Indonesia. A total of 27 semen samples from artificial insemination and breeding centers (Sembawa, Lembang, Ungaran,  and Sleman) in Indonesia have been examined and identified using the real-time PCR (qPCR) technique. The result showed that all samples were negative to BHV1. This indicated that semen from Ungaran, Sembawa, Sleman, and Lembang was safe to be used as a semen source for artificial insemination.
%R https://dx.doi.org/10.54203/scil.2021.wvj89
%N 4
%L eprints538