TY - JOUR SN - 23224568 (ISSN) AV - public TI - Detection of Bovine Herpesvirus 1 from Semen by Real-time PCR to Prevent the Spread of Infectious Bovine Rhinotracheitis Infection SP - 709 Y1 - 2021/12/25/ PB - Scienceline Publication, Ltd N2 - Infectious Bovine Rhinotracheitis (IBR) can be transmitted by livestock seeds and semen, through the process of artificial insemination. Therefore, it is necessary to detect the presence of the Bovine Herpesvirus 1 (BHV1) in semen through artificial insemination and breeding centers of Indonesia. The current study aimed to detect the presence of the virus in semen as a source of IBR disease transmission in Indonesia. A total of 27 semen samples from artificial insemination and breeding centers (Sembawa, Lembang, Ungaran, and Sleman) in Indonesia have been examined and identified using the real-time PCR (qPCR) technique. The result showed that all samples were negative to BHV1. This indicated that semen from Ungaran, Sembawa, Sleman, and Lembang was safe to be used as a semen source for artificial insemination. UR - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85123265800&doi=10.54203%2fscil.2021.wvj89&partnerID=40&md5=3d4921565277e16e1f19eaac3e85618f KW - Artificial insemination KW - Bovine Herpesvirus 1 KW - Infectious Bovine Rhinotracheitis KW - Real-time PCR KW - Semen IS - 4 EP - 712 VL - 11 ID - eprints538 JF - World's Veterinary Journal A1 - Untari, T. A1 - Kristianingrum, Y. P. A1 - Kusumawati, A. ER -