relation: http://eprints.science-line.com/id/eprint/538/ title: Detection of Bovine Herpesvirus 1 from Semen by Real-time PCR to Prevent the Spread of Infectious Bovine Rhinotracheitis Infection creator: Untari, T. creator: Kristianingrum, Y. P. creator: Kusumawati, A. subject: Q Science (General) subject: SF Animal culture description: Infectious Bovine Rhinotracheitis (IBR) can be transmitted by livestock seeds and semen, through the process of artificial insemination. Therefore, it is necessary to detect the presence of the Bovine Herpesvirus 1 (BHV1) in semen through artificial insemination and breeding centers of Indonesia. The current study aimed to detect the presence of the virus in semen as a source of IBR disease transmission in Indonesia. A total of 27 semen samples from artificial insemination and breeding centers (Sembawa, Lembang, Ungaran, and Sleman) in Indonesia have been examined and identified using the real-time PCR (qPCR) technique. The result showed that all samples were negative to BHV1. This indicated that semen from Ungaran, Sembawa, Sleman, and Lembang was safe to be used as a semen source for artificial insemination. publisher: Scienceline Publication, Ltd date: 2021-12-25 type: Article type: PeerReviewed format: text language: en identifier: http://eprints.science-line.com/id/eprint/538/1/WVJ%2011%284%29%20709-712%2C%20December%2025%2C%202021.pdf identifier: (2021) Detection of Bovine Herpesvirus 1 from Semen by Real-time PCR to Prevent the Spread of Infectious Bovine Rhinotracheitis Infection. World's Veterinary Journal. pp. 709-712. ISSN 23224568 (ISSN) relation: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85123265800&doi=10.54203%2fscil.2021.wvj89&partnerID=40&md5=3d4921565277e16e1f19eaac3e85618f relation: https://dx.doi.org/10.54203/scil.2021.wvj89