@article{eprints538, volume = {11}, year = {2021}, title = {Detection of Bovine Herpesvirus 1 from Semen by Real-time PCR to Prevent the Spread of Infectious Bovine Rhinotracheitis Infection}, publisher = {Scienceline Publication, Ltd}, author = {T. Untari and Y. P. Kristianingrum and A. Kusumawati}, journal = {World's Veterinary Journal}, month = {December}, number = {4}, pages = {709--712}, abstract = {Infectious Bovine Rhinotracheitis (IBR) can be transmitted by livestock seeds and semen, through the process of artificial insemination. Therefore, it is necessary to detect the presence of the Bovine Herpesvirus 1 (BHV1) in semen through artificial insemination and breeding centers of Indonesia. The current study aimed to detect the presence of the virus in semen as a source of IBR disease transmission in Indonesia. A total of 27 semen samples from artificial insemination and breeding centers (Sembawa, Lembang, Ungaran, and Sleman) in Indonesia have been examined and identified using the real-time PCR (qPCR) technique. The result showed that all samples were negative to BHV1. This indicated that semen from Ungaran, Sembawa, Sleman, and Lembang was safe to be used as a semen source for artificial insemination.}, url = {http://eprints.science-line.com/id/eprint/538/}, keywords = {Artificial insemination, Bovine Herpesvirus 1, Infectious Bovine Rhinotracheitis, Real-time PCR, Semen} }