TY  - JOUR
TI  - Polymorphism Association of Pituitary Positive Transcription Factor-1 Gene with Body Weight Traits in BC1 Hybrid Chicken (Gallus gallus gallus Linnaeus, 1758) from Cross Breeding between Female F1 Broiler and Male Pelung
SP  - 525
SN  - 23224568 (ISSN)
EP  - 532
KW  - Growth
KW  -  Hybrid chickens
KW  -  PIT-1 gene
KW  -  SNP
AV  - public
A1  - Retnosari, D.
A1  - Kilatsih, R.
A1  - Maulidi, I. S.
A1  - Trijoko, .
A1  - Daryono, B. S.
IS  - 4
PB  - Scienceline Publication, Ltd
JF  - World's Veterinary Journal
VL  - 10
Y1  - 2020/12/25/
ID  - eprints512
N2  - Pituitary Positive Transcription Factor-1 gene is closely related to chicken growth and productivity. This research was conducted to detect Single Nucleotide Polymorphism in the exon 6 Pituitary Positive Transcription Factor-1 gene and its association with the bodyweight growth in the first backcross hybrid chicken. Procedures of the research included crossbreeding female first filial broiler chicken with male Pelung chicken to obtain first backcross hybrid chicken, Day Old chick hatched were maintained during 49 days, the bodyweight on the Day-Old chick measured every seven days, DNA was isolated by Chelex 5% method, Pituitary Positive Transcription Factor-1 gene was amplified by PCR, DNA band was visualized utilizing electrophoresis, and the PCR product was sequenced using Sanger method. The DNA sequence was aligned using Clustal omega software to gain Single Nucleotide Polymorphism. The Single Nucleotide Polymorphism was analyzed using the Pearson correlation test between chicken body weights of 49-days-old chickens with the polymorphism points. The conclusion indicated that the bodyweight of the first backcross hybrid chicken was higher than the Pelung chicken but lower than the first filial broiler chicken. Single Nucleotide Polymorphism was not found on the exon 6 Pituitary Positive Transcription Factor-1 gene in the first backcross hybrid chicken.
UR  - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85099446324&doi=10.29252%2fscil.2020.wvj63&partnerID=40&md5=d9d0fd630d6a67c29cc981d4f5412a17
ER  -