eprintid: 508
rev_number: 9
eprint_status: archive
userid: 5
dir: disk0/00/00/05/08
datestamp: 2022-05-04 23:40:09
lastmod: 2022-05-04 23:40:17
status_changed: 2022-05-04 23:40:09
type: article
metadata_visibility: show
creators_name: Ramadan, R. M.
creators_name: Khalifa, M. M.
creators_name: Kamel, N. O.
creators_name: Abdel-Wahab, A. M.
creators_name: El-Bahy, M. M.
creators_id: putut.riyadi@live.undip.ac.id
title: The Use of Giardia immunogenic Protein Fraction to Distinguish Assemblages in Humans and Animals
ispublished: pub
subjects: Q1
subjects: SF
divisions: j13
full_text_status: public
keywords: Buffalo calves, Genotype, Giardia, Human, Immunoblot, Nested PCR
abstract: Buffalo calves have a high adverse effect on public health, specifically zoonotic Giardiasis in Egypt. The current study was carried out to investigate the use of enzyme-linked immunoelectrotransfer blot technique (EITB) as a preliminary step for the diagnosis of giardiasis before genotyping. For this purpose, fecal and blood samples were collected from diarrheic calves and workers in closed and non-closed beef buffalo farm. Zoonotic Giardia assemblage A was isolated from both diarrheic children and their close contact calves while the calves were infected by animal genotype assemblage E under low contact conditions. This was identified after the amplification and sequencing of a 292 bp fragment of 16S-rRNA ribosomal unit from 20 children and 28 calves Giardia isolates using nested PCR. Fractionation of different isolated Giardia assemblage and identification of specific fraction versus anti-Giardia-IgG antibodies in infected humans or animals using EITB revealed that α-1 giardin antigen with molecular weight ranged 29-34KDa. This specific immunogenic assemblage was fraction-related where it reacted specifically versus antibodies in sera of humans or animals infected by this assemblage and did not cross-react with giardin of other assemblage or with sera of non-infected cases. The results of the current study highlighted the use of EITB as a preliminary investigation before genotyping in the identification of zoonotic giardiasis.
date: 2020-09-25
publication: World's Veterinary Journal
volume: 10
number: 3
publisher: Scienceline Publication, Ltd
pagerange: 421-428
id_number: https://dx.doi.org/10.36380/scil.2020.wvj52
refereed: TRUE
issn: 23224568 (ISSN)
official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85096980954&doi=10.36380%2fscil.2020.wvj52&partnerID=40&md5=8c1d3aa9a0a7db7c6acdbd1b74b8550f
j_index: scopus
citation:    (2020)     The Use of Giardia immunogenic Protein Fraction to Distinguish Assemblages in Humans and Animals.   World's Veterinary Journal.  pp. 421-428.  ISSN 23224568 (ISSN)   
document_url: http://eprints.science-line.com/id/eprint/508/1/WVJ%2010%283%29%20421-428%2C%20Sep%2025%2C%202020.pdf